FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164411
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG59453.6159381063310847No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA55793.393325264124663No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA45242.751640705305606No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA39432.398258024098144No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT37452.277828125855326No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA37432.2766116622367116No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA37012.251065926245811No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC36122.196933295217473No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA35632.1671299365614223No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT35442.155573532184586No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG33512.0381847929883037No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG31741.930527762740936No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT31291.9031573313221135No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCCGAAAAA30881.8782198271405197No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT28111.7097396159624356No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC28061.7066984569158998No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC25481.5497746501146517No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC24651.499291409942157No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAATATCTAAAA23021.4001496250250896No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA22801.3867685252203321No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT22121.3454087621874449No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA21871.330202966954766No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT21451.3046572309638649No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT18341.115497138269337No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT15710.9555321724215533No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA15370.9348522909051098No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA15010.9129559457700519No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA14800.9001830777746014No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTACATTCCCACA14110.8582150829324071No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA13860.843009287699728No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA13810.8399681286531923No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA13640.8296281878949705No Hit
CCCTTTAAACTATCCCACCTTTCTAAACAAAACCAATATACATCTTAAAT12400.7542074435408823No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC11440.6958171898473946No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT10720.6520244995772788No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCAATT10460.6362104725352926No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA10410.6331693134887568No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA9960.6057988820699345No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC9760.5936342458837912No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT9300.5656555826556617No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC9030.5492333238043683No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA8560.5206464287669317No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA8310.5054406335342525No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC7690.4677302613572085No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACCAAACTACCTACCG7370.4482668434593793No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCAAA7230.43975159812907894No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA7130.4336692800360073No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT6590.4008247623334205No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT6420.3904848215751987No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACATAAATCC6020.3661555492029122No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT5720.3479085949236973No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCGA5150.313239381793189No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG5030.30594060008150303No Hit
TACCTTCCAAAAACTCTAAACATCCCCACACAACTACAAAACACCCAAAA4820.2931677320860526No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA4570.27796193685337356No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC4540.27613724142545204No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT4450.2706631551416876No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA4320.2627561416206945No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCGATCCCCTTCCTCCCAT4230.25728205533693No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC3760.22869516029949333No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA3700.22504576944365035No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCGAACC3590.21835521954127157No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT3530.2147058286854286No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT3420.2080152787830498No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3400.2067988151644355No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACCCTAA3170.19280948355037072No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAACCCCCCCAACAA3170.19280948355037072No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA3070.1867271654572991No Hit
ATATAATACCCACCTTCCAAACCACGAACCCTCAACCCAATCCTAAAAAC3030.18429423822007043No Hit
ATATCTCCTTACATCCCAACCTCGTCTATACAACAAAAAACCAAAAACTT3010.1830777746014561No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACTAAACCCCGAAAAA2880.17517076108046298No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC2590.15753203861055526No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT2530.15388264775471228No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCTAAAACTTCTCCATCTT2530.15388264775471228No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC2420.14719209785233348No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA2380.14475917061510482No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCTCCACAACCCGAACACTA2280.13867685252203318No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA2210.13441922985688307No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTTCCG2210.13441922985688307No Hit
CAAAACTAACTCTAAAATTTCCTTTTTCAATCTTAACCTAATAACCAACT2210.13441922985688307No Hit
CTATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT2160.13137807081034725No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCAAA2040.12407928909866127No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA1950.11860520281489681No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA1880.11434758014974666No Hit
CAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT1880.11434758014974666No Hit
AACTAATCTAAACCTCCTAACCTCAAACTATCTATCTACTTCTACCTCCG1810.11008995748459653No Hit
CAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC1810.11008995748459653No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC1670.10157471215429625No Hit
TAATACCAACTATACCACTCAACTCCTAAAAATTACCTTCCAAAAACTCT1660.10096648034498908No Hit
CACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG1650.10035824853568193No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCA1650.10035824853568193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)