FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170553
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG95935.624644538647811No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT60543.549629733865719No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA51243.0043446905067634No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA50302.9492298581672562No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT49172.8829747937591246No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA47562.788575985177628No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA44482.6079869600651997No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT39072.2907835101112264No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA38292.2450499258295076No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT35162.0615292606990203No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA31931.8721453155324153No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT30101.7648472908714592No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG29241.7144230825608464No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC28841.690969962416375No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT28701.68276137036581No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT28091.6469953621454914No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA27611.618851617972126No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC27431.6082977139071137No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA27291.600089121856549No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG26351.5449742895170415No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT23301.3661442484154485No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA22791.3362415202312479No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA22021.2910942639531406No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC21471.2588462237544928No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA20761.2172169354980564No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA20171.1826235832849612No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA19431.1392353110176894No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC18561.0882247747034646No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA15800.9263982457066132No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT15550.9117400456163186No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC14630.8577978692840349No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA14430.8460713092117992No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA12850.753431484641138No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC11630.6818994682005007No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA11370.6666549401065944No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCAAA10760.6308889318862758No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA9540.5593569154456386No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA9030.5294541872614378No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC8860.5194866112000376No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA8840.518313955192814No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC7830.4590948268280241No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA7120.4174655385715877No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG6940.40691163450657564No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCA6890.4039799944885168No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCAAAC6500.38111320234765733No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA6370.3734909383007042No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA6030.3535557861779037No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA5730.3359659460695502No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA5320.3119264979214672No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT5250.30782220189618476No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA5230.3066495458889612No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCAAACACTA5190.3043042338745141No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA4990.29257767380227845No Hit
ATATAATACCCACCTTCCAAACCACGAACCCACAACCCAATCCTAAAAAC4940.28964603378421955No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT4440.26032963360363054No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT4320.2532936975602892No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT4320.2532936975602892No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC4290.25153471354945384No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT4130.24215346549166533No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA4080.23922182547360646No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACGTCTACTCCG3500.20521480126412314No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT3480.20404214525689962No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA3350.1964198812099465No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT3210.18821128915938154No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCAA3210.18821128915938154No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC2910.17062144905102813No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA2820.1653444970185221No Hit
TAATACCAACTATACCACTCAACTCCTAAAAATTACCTTCCAAAAACTCT2750.16124020099323966No Hit
AAATAATAACATTAAATACTCCTCCCAAATACTCAAACTAAAACCCTTAA2670.15654957696434538No Hit
AAAAACTAACACACACTTATACCCCCAAAACAACCATAAATACACAAAAA2600.15244528093906293No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCGAACACTA2370.13895973685599197No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC2350.13778708084876842No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCAAA2300.13485544083070952No Hit
ATATAATACCCACCTTCCAAACCACGAACCCTCAACCCAATCCTAAAAAC2240.13133747280903882No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA2240.13133747280903882No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCTAAAACTTCTCCATCTT2160.1266468487801446No Hit
CCCAAAATCCTAAAATTCTCCTAACCTCAAAAACTCCCCACAAAAACAAA2100.12312888075847389No Hit
CCAAAACTCAACAACCTAAACCCGAAAATAACTACTAAACTACCTACCGC2080.12195622475125036No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCCATATTCCCACA2060.12078356874402679No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA2020.11843825672957967No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC2000.11726560072235609No Hit
TAAAACTCACTACTATCCCCCACAAACCAAAACTAAAAAACAAACTCTAA1990.11667927271874431No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCTCCACAACCCAAACACTA1980.11609294471513253No Hit
CTATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT1960.11492028870790899No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC1960.11492028870790899No Hit
CAAAACTAACTCTAAAATTTCCTTTTTCAATCTTAACCTAATAACCAACT1950.1143339607042972No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC1830.10729802466095584No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCAAACC1830.10729802466095584No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCGAACC1780.10436638464289692No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAACCCCCCCAACAA1770.10378005663928515No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCGA1760.10319372863567336No Hit
CCTAAAACAAACTAATACCACCCACTAAACTAAAACGAAACCCCGAAAAA1730.10143474462483804No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA1710.10026208861761447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)