FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000888031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84497
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG47225.588364083931974No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT35034.1457093151236135No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA31343.7090074203817887No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT28603.3847355527415175No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA27613.267571629762003No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG26593.146857284874019No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC23232.749210031125365No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA22002.6036427328780904No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA20892.4722771222646958No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA20802.4616258565392855No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA20212.391800892339373No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT17012.01308922210256No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT15401.8225499130146632No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC15171.7953300117163922No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT14991.7740274802655716No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA14981.7728440062960815No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA14411.7053859900351491No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA13371.582304697208185No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT12961.5337822644590933No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT12211.44502171674734No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA11961.4154348675100892No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC11371.3456099033101767No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT11231.329041267737316No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA9441.1171994271985988No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA8971.061576150632567No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA8581.0154206658224552No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC8360.9893842384936744No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA8260.977549498798774No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC6970.8248813567345586No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA6450.7633407103210764No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAACACAACCTAAATCC6430.7609737623820964No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT6280.7432216528397458No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT6050.7160017515414748No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG5650.6686627927618732No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA5500.6509106832195226No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCGAACC4950.5858196148975704No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC4830.5716179272636899No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT4670.5526823437518492No Hit
AAATAATAACATTAAATACTCCTCCCAACTAAAATAAACGTCTCCCCACC4520.5349302342094986No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA4130.488774749399387No Hit
AACTAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCG3670.43433494680284507No Hit
TAATACCAACTATACCACTCAACTCCTAAAAATTACCTTCCAAAAACTCT3350.39646377977916375No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCGA3210.37989514420630316No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC2750.3254553416097613No Hit
TAATACCAACTATACCACTCAACTCCGAGAAATTACCTTCCAAAAACTCT2610.3088867060369007No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA2550.30178586221996045No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT2530.2994189142809804No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA2460.2911345964945501No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCTCCACAACCCAAACACTA2410.2852172266470999No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATACTAAAAAC2330.2757494348911796No Hit
ATATAATACCCACCTTCCAAACCACGAACCCTCAACCCAATCCTAAAAAC2240.26509816916576917No Hit
ATATCTCCTTACATCCCAACCTCTCCTTCCTCTAATATTTTTGTAGAACC2180.25799732534882897No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2180.25799732534882897No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA2070.2449791116844385No Hit
CCAACCACTCCCTAAAAATCCCCCCAGAAGAACCTATTACATCTACTCCA2020.23906174183698828No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT1990.2355113199285182No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA1620.19172278305738666No Hit
CCCAAAATCCTAAAATTCTCCTAACCTCAAAAACTCCCCACAAAAACAAA1590.18817236114891653No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA1540.1822549913014663No Hit
CCTAAAACAAACTAATACCACCCACTAAACTAAAACGAAACCCTAAAAAA1500.17752109542350616No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC1500.17752109542350616No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCTAAACCCATTCGAA1450.17160372557605597No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA1430.16923677763707587No Hit
AACCCAATCTAAAACCAAAAACCTACTCATCCAATATAAAAAAAACAACT1300.15385161603370534No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACTAAACCCTAAAAAA1300.15385161603370534No Hit
AACCTTTATTTAATCAAAACTACTTAATTCGAAAAATATCCACGTTACTC1280.15148466809472527No Hit
CAAAACTAACTCTAAAATTTCCTTTTTCAATCTTAACCTAATAACCAACT1250.14793424618625511No Hit
AAAAACTAACACACACTTATACCCCCAAAACAACCATAAATACACAAAAA1240.14675077221676508No Hit
CCAAAACTCAACAACCTAAACCCGAAAATAACTACTAAACTACCTACCGC1180.13964992839982485No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA1140.13491603252186468No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT1090.12899866267441448No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA1040.12308129282696427No Hit
ATATAATACCCACCTTCCAAACCACTAACCCTCAACCCAATCCTAAAAAC1020.12071434488798419No Hit
CACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA990.11716392297951407No Hit
ATATCTCCTTACATCCCAACCCCTCCTTCCTCTAATATTTTTGTAGAACC990.11716392297951407No Hit
CAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT940.11124655313206386No Hit
CCAACCACTCCCTAAAAATCCCCCAAAAAAACCTATTACATCTACTCCGA930.11006307916257382No Hit
AACTAATCTAAACCTCCTAACCTCAGGTAATCTACCCATCTCAACCTCCC920.10887960519308379No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCAAC910.10769613122359374No Hit
AAAAACTAACACACACTTATACCCCCTAAACAACCATAAATACACAAAAA850.1005952874066535No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAACCCCCCCAACAA850.1005952874066535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)