FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151154
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG91766.070629953557299No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA43312.865289704539741No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT42152.7885467801050585No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT39732.628445161887876No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA39442.6092594307792054No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA38892.572872699366209No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA36572.4193868504968443No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC35252.332058695105654No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT33342.2056975005623403No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA32552.1534329227145825No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG32102.1236619606494043No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA31472.081982613758154No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA28961.9159268031279357No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT27771.8371991478889078No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC25331.675774375802162No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT25121.6618812601717454No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT24871.6453418368022017No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA24761.6380644905196025No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC23341.5441205657805945No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT22351.4786244492372018No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG20921.3840189475634121No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA19421.2847824073461502No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA19051.2603040607592257No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA18841.246410945128809No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA18741.2397951757809915No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT18461.2212710216071028No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC17011.1253423660637496No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA15741.0413220953464677No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT14280.9447318628683329No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA12540.8296174762163091No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA12020.7952154756076585No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA11670.7720602828902973No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC11150.7376582822816465No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT9790.6476838191513291No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT9360.619236010955714No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT9100.6020350106513886No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA8980.5940960874340078No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA8850.5854955872818449No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA8450.5590325098905752No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACCAACCCCGAA7970.5272768170210513No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCGAAA7940.5252920862167061No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA7580.5014753165645633No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACCCTAA7530.49816743189065454No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC7080.468396469825476No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCTAAAAAA7020.46442700821678556No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA5760.3810683144342855No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG5660.3744525450864681No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACCAACCCCGAAC5370.35526681397779747No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCATA4790.3168953517604562No Hit
TAATACCAACTATACCACTCAACTCCTAAAAATTACCTTCCAAAAACTCT4780.31623377482567444No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA4420.29241700517353164No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT4420.29241700517353164No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC4090.27058496632573403No Hit
CCCAAAATCCTAAAATTCTCCTAACCTCAAAAACTCCCCACAAAAACAAA3860.25536869682575386No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC3850.2547071198909721No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA3840.2540455429561904No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCAAA3700.24478346586924593No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA3500.231551927173611No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCGATCCCCTTCCTCCCAT3470.22956719636926579No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA3420.22625931169535704No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT3150.2083967344562499No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCA3140.20773515752146818No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT3100.20508884978234115No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC3030.200457811238869No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCGAA2960.19582677269539675No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCGAAA2790.18457996480410707No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTGTCTACTTCTACCTCCG2780.18391838786932532No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCTCCACGACCCGAACACTA2780.18391838786932532No Hit
ATATAATACCCACCTTCCAAACCACGAACCCTCAACCCAATCCTAAAAAC2690.1779641954562896No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC2600.17201000304325392No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACCAACCCCGA2510.16605581063021818No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT2470.16340950289109119No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACCAACCCCGAACC2430.16076319515196422No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA2150.14223904097807533No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA2150.14223904097807533No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA2120.1402543101737301No Hit
AACCCAATCTAAAACCAAAAACCTACTCATCCAATATAAAAAAAACAACT2090.13826957936938486No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA2020.13363854082591264No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCAAAC1960.12966907921722218No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCTAAAACTTCTCCATCTT1940.12834592534765868No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC1880.12437646373896821No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA1860.12305330986940471No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCTAAACCCATTCGAA1780.11776069439115075No Hit
CCAAAACTCAACAACCTAAACCCGAAAATAACTACTAAACTACCTACCGC1700.11246807891289677No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC1700.11246807891289677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)