FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000888035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103586
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCCACCG57625.562527754715888No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT47274.56335798273898No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA33893.271677639835499No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA33773.2600930627691005No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA32393.1268704265055125No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA31963.085359025350916No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG30202.9154518950437316No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC29302.8285675670457398No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT28122.7146525592261503No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT25902.50033788349777No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC25832.4935802135423706No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA23542.272507867858591No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA23192.238719518081594No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA22682.1894850655493987No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT22322.1547313343502017No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC18331.769544146892437No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA18321.7685787654702374No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT17111.651767613384048No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA15941.5388179869866585No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC15541.500202730098662No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT14371.3872531037012723No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC14051.3563608981908752No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCAA13911.3428455582800765No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCAAA11741.1333577896626958No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT11521.1121193983742979No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA10070.9721390921553106No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA9650.9315930724229143No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT8920.8611202286023207No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA7890.7616859421157299No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC7820.7549282721603305No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA7390.7134168710057344No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC7230.6979707682505357No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCGAATATCCT6090.5879172861197459No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCA5260.5077906280771532No Hit
CCAACCACTCCCTAAAAATCCTATAAAAAAATCACCCAACTACCTGTAGA4700.4537292684339582No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCTCCACAACCCAAACACTA4160.4015986716351631No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA4010.38711795030216434No Hit
CCCAAAATCCTAAAATTCTCCTAACCTCAAAAACTCCCCACAAAAACAAA3960.38229104319116486No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCGAACC3890.37553337323576547No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCAAAT3730.3600872704805669No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCGA3550.3427104048809685No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT3490.33691811634776897No Hit
ATATAATACCCACCTTCCAAACCACGGACCCGCAACCCAGTCCTAAAAAC3220.3108528179483714No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC3160.305060529415172No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA3150.304095147992972No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA3020.2915451895043732No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC2880.27802984959357446No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA2860.2760990867491746No Hit
AACCCAATCTAAAACCAAAAACCTACTCATCCAATATAAAAAAAACAACT2380.22976077848357884No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT2290.22107234568377968No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA2250.21721081999498001No Hit
ATATAATACCCACCTTCCAAACCACGAACCCTCAACCCAATCCTAAAAAC2230.21528005715058018No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC2140.206591624350781No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT2100.20273009866198133No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCTAAACCCATTCGAA1890.18245708879578323No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA1780.17183789315158418No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAGA1740.16797636746278455No Hit
CACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA1690.163149460351785No Hit
AACTAATCTAAACCTCCTAACCTCAAACTATCTATCTACTTCTACCTCCG1650.15928793466298535No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCTAAAACTTCTCCATCTT1610.1554264089741857No Hit
CTATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT1570.15156488328538606No Hit
CCCAAAATCCTAAAATTCTCCTAACTGCAAAAACTCCCCACAAAAACAAA1460.14094568764118703No Hit
CACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG1420.13708416195238737No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC1350.130326491996988No Hit
CCCTTTAAACTATCCCACCTTTCTAAACTAAACCAATATACATCTTAAAT1350.130326491996988No Hit
CAAAACTAACTCTAAAATTTCCTTTTTCAATCTTAACCTAATAACCAACT1330.12839572915258818No Hit
AAAAACTAACACACACTTATACCCCCTAAACAACCATAAATACACAAAAA1320.12743034773038828No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAACCCCCCCAACAA1250.1206726777749889No Hit
CCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC1200.11584577066398934No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACTAAAAATA1180.11391500781958953No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT1180.11391500781958953No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCAAC1130.10908810070858997No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT1120.10812271928639006No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTCCCTCCCAT1120.10812271928639006No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAAACCCCCCC1110.10715733786419014No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACCCCCCACAAAAACAAA1110.10715733786419014No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA1090.10522657501979032No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAAATTAAACAAAACGTCTAAA1080.10426119359759041No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCTAA1060.10233043075319058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)