FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163870
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGACGGTGAGG50683.0926954293037165No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGTGGTGG48732.973698663574785No Hit
GGTAGGAGGGGTTGTATAAGGTTTATTTGGAAGTTAGAATTTATTTGTGG43392.647830597424788No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTTAAAGAG43092.6295234026972603No Hit
TTGTGGGGATTTAGGTTGTATTATGCGGATGAGGTTTTTAGGTAGTAGGT42942.620369805333496No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAG42792.611216207969732No Hit
TGGGGAGGGAAGAGTAGATTTTACGATATTTTTGGGGTTTTTAGATAGTT41552.5355464697626164No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTC40682.4824556050527855No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTCGTTGACGGTTTT40452.4684200890950145No Hit
TAGAAAGGTGGGATAGTTTAAAGGGGGATTTTTAGGTTATAGGTAAATTT38992.3793250747543784No Hit
AAAGAGATTTATTTTGAGTGATTATGGTTCGTGATATAGTTTTAGGAGAT38372.341490205650821No Hit
GAAGTTGTGGAATTTAGGTAGGAAGTGGTTGATTTTTAGTGTTTAGTTGT37212.270702386037713No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTTATAGTAGATTGGAGGA35552.1694025752120583No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTACGTTTTGTTGT33642.052846768780131No Hit
GGTTTGGAAGGTGGGTATTATATTTTGTTTTGTTTTTGAAGTTTAGTTTT33502.0443034112406178No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT32501.9832794288155244No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTCGGTTTTTTTTGTTTGTTTGT31121.899066333068896No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTCGGAAGGGAG30021.8319399524012938No Hit
GTTTGGAGGAGTTGGTTTTTTAGAGTTGGTGTTGTTGGTGTTGTATTTTG29971.828888753280039No Hit
GTTGGGATTTTAGTTTATTTTTGTAATTTAAATATTTTTTAGTAAAGTTT29681.811191798376762No Hit
TTGAGGTTATTAGAAGGGAGGTTTTAGTTATATAGGGTGGAAGAATTTAA29381.7928846036492343No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTA25881.5793006651614083No Hit
AAAATATTAGAGGAAGGAGGGGTTTGGGTTTCGTTGGTTTTGTTTAAAAA24971.5237688411545738No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTCGGGGTTTCGTTTTCGTTTAG24601.5011899676572893No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTTAGGGTTTCGTTTTCGTTTAG23261.4194178312076646No Hit
TGTTTGTTTTGGGATTTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTA22751.388295600170867No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTTGAGGAAATTAAG22401.3669372063220846No Hit
GGTTTGGAAGGTGGGTATTATATTTTGTTTTGTTTTCGAAGTTTAGTTTT17221.0508329773601026No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGCG13770.8403002379935314No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTATTT13090.7988039299444681No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGTG11680.7127601147250869No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTTAT11610.7084884359553304No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTACGGAGGAGAGGGGTTT11600.7078781961310795No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTTGGAGGTGTTATGTTTTGTTGT11300.6895710014035517No Hit
GTTTGGAGGAGTTGGTTTTTTAGAGTTGGCGTTGTTGGTGTTGTATTTTG11240.685909562458046No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGCG11070.6755354854457802No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTTAT10960.66882284737902No Hit
ATTATTTTTTTAGGGAGTTGGGTTGTATTTTATTTGTTGTTTTGTTTTTA9810.598645267590163No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTTGGTTTTTTTTGTTTGTTTGT9800.598035027765912No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGTG7870.4802587416854824No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTATGGAGGAGAGGGGTTT7860.4796485018612314No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTTGTTGATGGTTTT7650.4668334655519619No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTTA7400.45157746994568865No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGT6950.4241166778543967No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTT6920.42228595838164396No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTATAGTAGATTGGAGGAG5630.34356502105327397No Hit
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGATGGTGAGG4990.3045096723012144No Hit
TGGGGAGGGAAGAGTAGATTTTATGATATTTTTGGGGTTTTTAGATAGTT4820.29413559528894856No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTTGGGTGTAGGGGATAA4800.2929151156404467No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTATT4590.28010007933117714No Hit
AAAATATTAGAGGAAGGAGGGGTTTGGGTTTTGTTGGTTTTGTTTAAAAA4550.2776591200341734No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGCGCGGT4520.2758284005614206No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTAG4460.272166961615915No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTGGGTGTAGGGGATAAG3860.2355525721608592No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTATT3860.2355525721608592No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTCGTGGTG3840.23433209251235734No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTTGTTGACGGTTTT3780.23067065356685176No Hit
GTTGTGGTTATTGGTTTTGTGTCGTTTTTTTTTTCGGGTGTAGGGGATAA3700.22578873497284432No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTTGGTTTGTTTTTAATTTTTTAG3670.22395801550009153No Hit
GAGAGTAGTAGGAGGAGGAGGAGGGAAATAGGAAATTTTAGGTTTTGGTT3640.22212729602733874No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGCG3490.2129736986635748No Hit
AAAGAGATTTATTTTGAGTGATTATGGTTTGTGATATAGTTTTAGGAGAT3410.20809178006956733No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTTAT3370.2056508207725636No Hit
AAAATATTAGAGGAAGGAGGGGTTTGGGTTTCGTTGGTTTTGTTTAAAAG3350.20443034112406175No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTCGTGGCG3120.19039482516629036No Hit
GGTTGGAGTTTTAGGTGTATTTGGGTGTAGGTTTTTTTGAGGAAATTAAG3080.18795386586928664No Hit
TGTTTGTTTTGGGATTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTAT3060.18673338622078478No Hit
TGGGTTTTAGATATTTGTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAGG3030.184902666748032No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGCTCGGGTTGTGGTG3000.18307194727527917No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTTAGGGTTTCGTTTTTGTTTAG2990.18246170745102824No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750.16781595166900592No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATTGTCGTTTTTTTTTA2720.16598523219625314No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTCGGTTTTTTTGTTTGTTTGTG2670.16293403307499846No Hit
GTTGTGGTTATTGGTTTTGTGTCGTTTTTTTTTCGGGTGTAGGGGATAAG2620.1598828339537438No Hit
GAGAGTAGTAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGTAGG2500.15255995606273265No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTCTGGGTGTAGGGGATAA2370.14462683834747053No Hit
TTGTGGGGATTTAGGTTGTATTATGCGGATGAGGTTTTTAGATAGTAGGT2370.14462683834747053No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTTTA2290.1397449197534631No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGCGTGGT2140.13059132238969914No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTCGTGGTGG2140.13059132238969914No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTAGGGTTTCGTTTTCGTTTAGT2050.12509916397144077No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGGGGTAAGCGAGAGGGGGTGCGGT2050.12509916397144077No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTTTGGGTGTAGGGGATA2010.12265820467443704No Hit
TGTTTGTTTTGGGATTTTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGT1970.12021724537743332No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTCGGGTGTAGGGGATAA1890.11533532678342588No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTTTA1880.11472508695917495No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGTG1880.11472508695917495No Hit
AAAATATTAGAGGAAGGAGGGGTTTGCGTTTCGTTGGTTTTGTTTAAAAA1870.11411484713492402No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGCG1850.11289436748642216No Hit
GAAGTTGTGGAATTTAGGTAGGAGTGGGGGTTGGGAGAGACCAAGTCTCT1760.10740220906816379No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTTT1670.10191005064990541No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)