FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163870
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA49803.0389943247696345No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG49273.006651614084335No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT44342.7058033807286264No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA43462.6521022761945447No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG39662.420211142979191No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA39222.39336059071215No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA37942.3152498932080308No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA36432.2231036797461403No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT32151.9619210349667418No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT31941.9491059986574724No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC30441.8575700250198328No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT28531.7410142185879052No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC28031.7105022273753587No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG27301.6659547202050404No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC26241.601269298834442No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT26151.5957771404161836No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT25341.5463477146518583No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT25141.5341429181668396No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA25111.5323121986940869No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC24781.5121742844938062No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA24421.4902056508207726No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT21731.3260511380972724No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA21591.3175077805577593No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA21431.3077439433697442No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA20821.2705193140904374No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA19151.1686092634405323No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA18951.1564044669555134No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA18491.1283334350399707No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA15670.9562458046012082No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC14930.9110880576066395No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCCAAACCA14570.889119423933606No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT13160.8030756087142247No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA12920.7884298529322024No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA12530.7646304997864161No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA12410.7573076218954049No Hit
ATATAATACCCACCTTCCAAACCACGAATCCGCAACCCAATCCTAAAAAC10650.649905412827241No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT9590.5852199914566425No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCAAA9350.5705742356746201No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA9250.5644718374321108No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA8570.5229755293830476No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT8410.5132116921950327No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA8300.5064990541282725No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA8140.49673521694025746No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC7930.48392018063098796No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG7890.48147922133398424No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC7150.4363214743394154No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT6990.42655763715140055No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC6920.42228595838164396No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA6580.40153780435711234No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCAAAC6370.3887227680478428No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT6300.3844510892780863No Hit
ATATAATACCCACCTTCCAAACCACAAATCCGCAACCCAATCCTAAAAAC5630.34356502105327397No Hit
CCCTTTAAACTATCCCACCTTTCTAAACAAAACCAATATACATCTTAAAT5260.3209861475559895No Hit
ATATCTCCTTACATCCCAACCTCGTCTATACAACAAAAAACCAAAAACTT5120.31244279001647646No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA5100.3112223103679746No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCAAACACTA4810.29352535546469766No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCGAAA4660.2843717581009336No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT4280.2611826447793983No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA4230.25813144565814367No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3850.23494233233660827No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA3770.23006041374260083No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA3680.22456825532434246No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT3660.2233477756758406No Hit
AAATAATAACATTAAATACTCCTCCCAAACACACACCGAAAAAAATAAAC3490.2129736986635748No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA3440.20992249954232012No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCGAAA3360.20504058094831268No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC3310.20198938182705803No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT3300.2013791420028071No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCGAACC3050.18612314639653385No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCAA3030.184902666748032No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCAAAA3010.18368218709953013No Hit
CACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG2940.1794105083297736No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2750.16781595166900592No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC2620.1598828339537438No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACCAAACTACCTACCG2620.1598828339537438No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCGA2600.15866235430524195No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2510.15317019588698358No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCAATT2450.149508756941478No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCTAAAACTTCTCCATCTT2430.14828827729297614No Hit
AACTAATCTAAACCTCCTAACCTCAAACTATCTATCTACTTCTACCTCCG2370.14462683834747053No Hit
TAATACCAACTATACCACTCAACTCCTAAAAATTACCTTCCAAAAACTCT2360.1440165985232196No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATACACATCTTAAAT2350.14340635869896867No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT2340.14279611887471774No Hit
CAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC2330.1421858790504668No Hit
CTATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT2270.13852444010496123No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAGA2230.1360834808079575No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCAAACC2220.13547324098370658No Hit
CAAAACTAACTCTAAAATTTCCTTTTTCAATCTTAACCTAATAACCAACT2170.13242204186245193No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA2140.13059132238969914No Hit
CAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT2090.1275401232684445No Hit
CCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC2060.1257094037956917No Hit
CAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC2010.12265820467443704No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC2010.12265820467443704No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCTAAACCCATTCGAA2010.12265820467443704No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA1950.11899676572893146No Hit
AACCCAATCTAAAACCAAAAACCTACTCATCCAATATAAAAAAAACAACT1930.1177762860804296No Hit
AAAAACTAACACACACTTATACCCCCTAAACAACCATAAATACACAAAAA1840.11228412766217123No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT1830.1116738878379203No Hit
CAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA1820.11106364801366937No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCTAA1740.10618172941966193No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC1720.10496124977116006No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA1710.10435100994690913No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACTAAACCCCGAAAAA1680.10252029047415634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)