FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160147
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGACGGTGAGG70744.41719170512092No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTTAGGGTTTCGTTTTCGTTTAG53013.3100838604532083No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGTGGTGG52263.2632518873285172No Hit
TTGTGGGGATTTAGGTTGTATTATGCGGATGAGGTTTTTAGGTAGTAGGT44832.7993031402399047No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTTAAAGAG42362.6450698420825867No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTA42222.6363278737659774No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTCGGAAGGGAG39912.492085396541927No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTCGTTGACGGTTTT37662.351589477167852No Hit
GTTTGGAGGAGTTGGTTTTTTAGAGTTGGTGTTGTTGGTGTTGTATTTTG36452.276033893860016No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTTGGTTTTTTTTGTTTGTTTGT35082.1904874896189126No Hit
GGTAGGAGGGGTTGTATAAGGTTTATTTGGAAGTTAGAATTTATTTGTGG34942.181745521302304No Hit
TGGGGAGGGAAGAGTAGATTTTACGATATTTTTGGGGTTTTTAGATAGTT34802.1730035529856946No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTTATAGTAGATTGGAGGA33352.0824617382779573No Hit
TAGAAAGGTGGGATAGTTTAAAGGGGGATTTTTAGGTTATAGGTAAATTT32382.0218923863700224No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTACGTTTTGTTGT31901.9919199235702199No Hit
GTTTGGAGGAGTTGGTTTTTTAGAGTTGGCGTTGTTGGTGTTGTATTTTG28381.7721218630383335No Hit
AAAGAGATTTATTTTGAGTGATTATGGTTCGTGATATAGTTTTAGGAGAT26561.6584762749224151No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTTGGTTTGTTTTTAATTTTTTAG26541.6572274223057566No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGTG25991.6228839753476494No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAG24541.5323421606399121No Hit
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGATGGTGAGG21691.3543806627660837No Hit
AAAATATTAGAGGAAGGAGGGGTTTGGGTTTTGTTGGTTTTGTTTAAAAA21651.3518829575327667No Hit
GTTGGGATTTTAGTTTATTTTTGTAATTTAAATATTTTTTAGTAAAGTTT21011.3119196737996965No Hit
GGGAGATTAAGTAGGTGAGTGGTTTGTTTCGAGTTTGGTTTTTTTTTATT19571.2220022854002885No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTTGAGGAAATTAAG17971.1220940760676128No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGT17871.1158498129843206No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTACGGAGGAGAGGGGTTT17861.1152253866759914No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGCG17521.0939948921927978No Hit
TTGAGGTTATTAGAAGGGAGGTTTTAGTTATATAGGGTGGAAGAATTTAA17041.0640224293929952No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGCGGTGG16991.060900297851349No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTC16861.0527827558430691No Hit
AAAATATTAGAGGAAGGAGGGGTTTGGGTTTCGTTGGTTTTGTTTAAAAA16801.0490361979930938No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGCG16751.0459140664514477No Hit
TGTTTGTTTTGGGATTTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTA14470.9035448681523849No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTCGGTTTTTTTTGTTTGTTTGT13350.8336091216195121No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTTAT12900.805509937744697No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT12160.7593023909283345No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTTGTTGATGGTTTT10200.636914834495807No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTT9030.5638569564212879No Hit
GAAGTTGTGGAATTTAGGTAGGAAGTGGTTGATTTTTAGTGTTTAGTTGT8880.5544905617963496No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTTCGGGCGTAGGGGATA8450.527640230538193No Hit
ATTATTTTTTTAGGGAGTTGGGTTGTATTTTATTTGTTGTTTTGTTTTTA8350.5213959674549008No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTTGGGTGTAGGGGATAA8050.5026631782050242No Hit
GAAGTTGTGGAATTTAGGTAGGAATTTAGGAGTGGGGGTTGGGAGAGACC7720.4820571100301598No Hit
GGTTTGGAAGGTGGGTATTATATTTTGTTTTGTTTTTGAAGTTTAGTTTT7590.4739395680218799No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTGGGTGTAGGGGATAAG7370.460202189238637No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGCG7140.4458403841470649No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTCGCTGACGGTTTT6890.4302297264388343No Hit
GGGGATTGGGATAAAGGAGTATTATTTTTGGTTTTTGTTTGAGTGATTTC6890.4302297264388343No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTCGGGCGTAGGGGATAA6820.4258587422805298No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTAG5870.3665382429892536No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTATT5660.3534252905143399No Hit
GAGAGTAGTAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGTAGG5590.3490543063560354No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTT5490.34281004327274317No Hit
GAAGTTGTGGAATTTAGGTAGGAGTGGGGGTTGGGAGAGACCAAGTCTCT5360.3346925012644633No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTTA4800.2997246279980268No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTAGGGTTTCGTTTTCGTTTAGT4800.2997246279980268No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGCG4050.2528926548733351No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGTG3360.20980723959861877No Hit
GGTAGGAGGGGTTGTATAAGGTTTATTTGGAAGTTAGGATTTATTTGTGG3310.20668510805697266No Hit
AAAGAGATTTATTTTGAGTGATTATGGCTCGTGATATAGTTTTAGGAGAT3260.20356297651532657No Hit
GAAGTTGTGGAATTTAGGTAGGAAGTGGTTGATTTTTAGTCTTTAGTTGT3130.19544543450704666No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTGGGTGTAGGGGATAAGA2930.18295690834046222No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTATAGTAGATTGGAGGAG2920.18233248203213298No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTTGGTTTTTTTGTTTGTTTGTG2900.18108362941547454No Hit
GGGAGATTAAGTAGGTGAGTGGTTTGTTTCGAGTTTGGTTTTTTTTATTT2880.1798347767988161No Hit
GGATTTTTTTGATTTTTTTGTTTTGGTTAAATAGAAATAGTATTGTTTAT2730.17046838217387775No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGGGGTAAGCGAGAGGGGGCGTGGT2710.1692195295572193No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2630.1642241190905855No Hit
TGTTTGTTTTGGGATTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTAT2530.1579798560072933No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTTAT2530.1579798560072933No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTTTCGGGCGTAGGGGAT2470.15423329815731798No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTTTGGGTGTAGGGGATA2420.15111116661567184No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTATGGAGGAGAGGGGTTT2340.14611575614903807No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTCGGGCGTAGGGGATAAG2270.14174477199073351No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTTTA2060.12863181951581984No Hit
GGGAGATTAAGTAGGTGAGTGGTTTGTTTCGAGTTTGGTTTTTTTTTTAT1860.1161432933492354No Hit
AAAATATTAGAGGAAGGAGAGGTTGGGATGTAAGGAGATATAGACCAAGT1840.11489444073257696No Hit
GGTTGGAGTTTTAGGTGTATTTGGGTGTAGGTTTTTTTGAGGAAATTAAG1790.11177230919093083No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTATTT1720.10740132503262627No Hit
TGGGTTTTAGATATTTGTTTTTTTGGTTGGTTTGTTTTTAATTTTTTAGG1680.10490361979930939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)