FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160147
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG56453.524886510518461No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA55583.4705614216938185No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC47682.977264638113733No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT44502.7786970720650404No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA43492.715630014923789No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG41072.5645188483081167No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA40462.5264288435000344No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA39862.488963265000281No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT37222.3241147196013663No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT36112.2548033993768226No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT35572.2210843787270442No Hit
CCAAAACTCAACAACCTAAACCCCAAAAATAACTACTAAACTACCTACCG34412.1486509269608547No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA33752.107438790611126No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC28861.8020943258381363No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC28391.7727462893466626No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT28031.7502669422468107No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA26241.63849463305588No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA25671.6029023334811143No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT25631.6004046282477973No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA24051.5017452715317803No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT23091.4418003459321749No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACAAAACACCCAAAA22861.4274385408406027No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA22241.388724109724191No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA22081.3787332887909234No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG21581.347511973374462No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA21421.3375211524411945No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT20511.2806983583832354No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC20511.2806983583832354No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT20481.2788250794582476No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA18921.1814145753588892No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA16761.046538492759777No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA14670.9160333943189695No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTGAAA13940.8704502738109361No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA11820.738071896445141No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCGATCCCCTTCCTCCCAT11290.7049773021036922No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA11120.6943620548620955No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA10240.6394125397291238No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA9460.5907072876794446No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC9450.5900828613711152No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC8640.5395043303964483No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA8440.5270158042298638No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA8160.5095318675966456No Hit
CTCCCAACCCCCACTCCTAAATTCCTACCTAAATTCCACAACTTCTGTAG7760.48455481526347666No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC7560.47206628909689224No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG6940.4333518579804805No Hit
CCCTTTAAACTATCCCACCTTTCTAAACAAAACCAATATACATCTTAAAT6440.40213054256401937No Hit
AACTAATCTAAACCTCCTAACCTCAAGCGATCTATCTACTTCTACCTCCG6020.37590463761419196No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT5540.34593217481438926No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA5480.34218561696441396No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC5130.32033069617289117No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT5050.31533528570625735No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT4650.29035823337308847No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT4530.2828651176731378No Hit
CCCAAAATCCTAAAATTCTCCTAACCTCAAAAACTCCCCACAAAAACAAA4380.27349872304819944No Hit
ATATAATACCCACCTTCCAAACCACGAACCCTCAACCCAATCCTAAAAAC3990.2491460970233598No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA3820.238530849781763No Hit
AACTAATCTAAACCTCCTAACCTCAAATAACCCGCCTACCTCGACCTCCC3770.2354087182401169No Hit
TAATACCAACTATACCACTCAACTCCTAAAAATTACCTTCCAAAAACTCT3740.23353543931512924No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT3150.1966942871237051No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC3050.19045002404041286No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA3030.18920117142375442No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT2960.18483018726544986No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC2910.18170805572380377No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA2810.1754637926405115No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA2630.1642241190905855No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA2530.1579798560072933No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2390.1492378876906842No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACTAAACCCTAAAAAA2300.1436180509157212No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC2180.13612493521577052No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCTAAAACTTCTCCATCTT2120.13237837736579516No Hit
CCTAAAACAAACTAATACCACCCACTAAACTAAAACGAAACCCTAAAAAA2090.13050509844080752No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCAAA2030.1267585405908322No Hit
CTATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT2030.1267585405908322No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC2020.12613411428250296No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT2010.12550968797417372No Hit
ATATAATACCCACCTTCCAAACCACTAACCCTCAACCCAATCCTAAAAAC1970.12301198274085683No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCTAAACCCATTCGAA1970.12301198274085683No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAACCCCCCCAACAA1970.12301198274085683No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCTCCACAACCCGAACACTA1800.11239673549926005No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA1790.11177230919093083No Hit
CCAAAACTCAACAACCTAAACCCGAAAATAACTACTAAACTACCTACCGC1760.10989903026594317No Hit
ATATCTCCTTACATCCCAACCTCTCCTTCCTCTAATATTTTTGTAGAACC1750.10927460395761393No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA1680.10490361979930939No Hit
AAAAACTAACACACACTTATACCCCCTAAACAACCATAAATACACAAAAA1640.1024059145659925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)