FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000888041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116775
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG48504.15328623421109No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA43823.7525155213016483No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA41173.5255833868550637No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA37913.246414044101906No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT37813.237850567330336No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT37453.2070220509526868No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA30702.628987368871762No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC28192.4140441019053736No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG26222.245343609505459No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT24482.096339113680154No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT24142.0672232926568186No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA23942.05009633911368No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT20691.7717833440376793No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT20281.7366730892742452No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT19261.6493256262042388No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA18691.600513808606294No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC17181.4712053093555983No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG17091.4634981802611862No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA17081.4626418325840291No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA16211.3881395846713767No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC16091.3778634125454934No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACCACCTACCG15221.303361164632841No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA14911.2768143866409762No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA14401.2331406551059731No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT13641.1680582316420467No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC12761.0926996360522372No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA11991.026760864911154No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA11981.0259045172339971No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA10420.8923142795975166No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC10350.8863198458574182No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCGAAA9980.8546349818026119No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA9550.8178120316848639No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA9440.8083922072361379No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA9370.8023977734960395No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA9040.7741383001498608No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA8860.7587240419610362No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA8510.7287518732605438No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCAAA6890.5900235495611218No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT6610.5660458146007279No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCGATCCCCTTCCTCCCAT6590.564333119246414No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA6110.5232284307428816No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCGAA5500.47099122243630914No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA5120.43845001070434597No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC5050.43245557696424747No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG4910.42046670948405046No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA4550.3896381931064012No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT4500.3853564547206166No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC4140.35452793834296725No Hit
AACTAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCG4120.3528152429886534No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA4080.34938985228002567No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCAAAC4070.3485335046028688No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCAAAA3640.31171055448512097No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC3380.2894455148790409No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA3190.2731749090130593No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC3060.26204238921001927No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT2790.23892100192678228No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2320.19867266110040674No Hit
ATATAATACCCACCTTCCAAACCACGAACCCTCAACCCAATCCTAAAAAC2310.19781631342324987No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT2300.19695996574609292No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCAAA2280.19524727039177905No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT2270.19439092271462213No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCAA2170.1858274459430529No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCGA2140.1832584029115821No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAGACAAA2090.17897666452579747No Hit
AACAACAATATTTCCTAATCCTCCCAACTAGAAAAAAAAAACCCCCCCAA2020.172982230785699No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC1920.16441875401412975No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA1750.149860843502462No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA1710.1464354527938343No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAGCAAAACATCTAAAA1700.14557910511667738No Hit
AAATAATAACATTAAATACTCCTCCCAATCCCTCCTCCACCACCTAATCC1700.14557910511667738No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA1680.1438664097623635No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC1650.14129736673089274No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC1580.13530293299079427No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACTAAACCCTAAAAAA1560.1335902376364804No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCAAACC1540.13187754228216655No Hit
CCTAAAACAAACTAATACCACCCACTAAACTAAAACGAAACCCTAAAAAA1530.13102119460500963No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCTAAAACTTCTCCATCTT1480.126739456219225No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCTCCACAACCCGAACACTA1440.12331406551059731No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCGAACC1420.12160137015628344No Hit
TAATACCAACTATACCACTCAACTCCTAAAAATTACCTTCCAAAAACTCT1410.12074502247912654No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCTAA1220.10447441661314494No Hit
ATATAATACCCACCTTCCAAACCACTAACCCTCAACCCAATCCTAAAAAC1210.10361806893598802No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCTAA1200.10276172125883108No Hit
AAAAACTAACACACACTTATACCCCCTAAACAACCATAAATACACAAAAA1170.1001926782273603No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT1170.1001926782273603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)