FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000888045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67256
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG39035.803199714523611No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA27964.157249910788628No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT26623.9580111811585583No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA23213.450993219935768No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA23023.4227429523016535No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT21503.1967408112287377No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG19422.8874747234447486No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA12861.912097061972166No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC11811.7559771618889022No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT11791.7530034495063636No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT11681.7366480314024029No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA11241.6712263589865588No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC10871.6162126799095993No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT10651.5835018437016772No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT10251.52402759605091No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT9601.4273819436184132No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG9541.4184608064707982No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC9381.3946711074104912No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT9281.3798025454977996No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA9141.358986558820031No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA9121.3560128464374925No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA8951.3307362911859164No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA8491.2623409063875342No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA8371.244498632092304No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCAAAA7631.1344712739383847No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC7521.1181158558344237No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT7001.0407993338884263No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC6801.0110622100630426No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA6360.9456405376471988No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA6040.898061139526585No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA6000.8921137147615082No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA5980.8891400023789698No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC5800.8623765909361246No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA5290.7865469251813964No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA5120.7612703699298203No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA5050.7508623765909361No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT4110.6110978946116331No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA3470.5159390983704056No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA3460.5144522421791364No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC3450.5129653859878672No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA3240.4817414059712145No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC3160.469846556441061No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT2770.41185916498156294No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA2700.4014511716426787No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG2690.3999643154514096No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCAAACACTA2480.36874033543475676No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA2360.35089806113952654No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCGAAA2320.3449506363744499No Hit
TAATACCAACTATACCACTCAACTCCTAAAAATTACCTTCCAAAAACTCT2270.337516355418104No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC2130.31670036874033547No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA1980.29439752587129775No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT1920.28547638872368264No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT1900.2825026763411443No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA1880.27952896395860594No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA1820.2706078268109909No Hit
AACTAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTGCCTCCG1700.25276555251576066No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA1510.22451528488164624No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATCA1450.21559414773403116No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC1320.19626501724753181No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCGA1300.19329130486499346No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCGAACC1220.18139645533484003No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT1180.17544903056976327No Hit
AACCCAATCTAAAACCAAAAACCTACTCATCCAATATAAAAAAAACAACT1180.17544903056976327No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA1120.16652789342214822No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC1100.16355418103960984No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC1090.16206732484834066No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT1090.16206732484834066No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCTCCACGACCCGAACACTA1000.14868561912691802No Hit
ATATAATACCCACCTTCCAAACCACGAACCCTCAACCCAATCCTAAAAAC1000.14868561912691802No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT990.14719876293564887No Hit
CCTAAAACAAACTAATACCACCCACTAAACTAAAACGAAACCCTAAAAAA980.14571190674437967No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACTAAACCCTAAAAAA970.1442250505531105No Hit
TAAAACTCACTACTATCCCCCACAAATACACCTAAAACTCCAACCTGTAG940.13976448197930297No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAACCTATTACATCTACTCCGA940.13976448197930297No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCTAAACCCATTCGAA910.13530391340549544No Hit
AAAAACTAACACACACTTATACCCCCTAAACAACCATAAATACACAAAAA900.13381705721422624No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCTCCACAACCCGAACACTA900.13381705721422624No Hit
ACACTCTAAAATTTCCCCTAAAAAACTGGTTCAAGCCATGACGGGAAGTG880.13084334483168789No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAGAAACTCT840.12489592006661115No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACTTTCCAAAAACTCT820.1219222076840728No Hit
CCAAAACTCAACAACCTAAACCCGAAAATAACTACTAAACTACCTACCGC750.11151421434518853No Hit
ATATAATACCCACCTTCCAAACCACAAACCCTCAACCCAATCCTAAAAAC730.10854050196265018No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCTAA720.10705364577138099No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAGCCATAACGAAAAATA710.10556678958011181No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT710.10556678958011181No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCTAAAACTTCTCCATCTT700.10407993338884262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)