FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191768
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG88874.634245546702266No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA76253.9761586917525342No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA60443.1517250010429274No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG52902.758541571065037No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA51282.674064494597639No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT44362.313211797588753No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT41122.1442576446539565No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC38672.016499103082892No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA38011.982082516373952No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA32731.706749822702432No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT32371.6879771390430103No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT32121.6749405531684118No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT31171.6254015268449378No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT31051.6191439656251305No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC29931.5607400609069293No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA29721.5497893287722666No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT27591.4387176171206875No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA25761.3432898085186267No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA24271.2655917567060198No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA23891.2457761461766301No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCGAACACTA23421.2212673647323848No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT22931.195715656418172No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA22591.177985899628718No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA22361.1659922406240875No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG21961.14513370322473No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC21771.1352258979600351No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT21691.1310541904801634No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC20191.0528346752325728No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT20141.050227358057653No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA20111.048662967752701No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT17790.9276834508364273No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA17120.8927454006925035No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC17070.8901380835175837No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCA16810.8765800342080012No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA14220.7415210045471612No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA13400.6987610028784782No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCAAA13020.6789453923490885No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCAAA13000.6779024654791206No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT11830.6168912435859998No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC11440.5965541696216261No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT11130.580388803137124No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA9760.5089483125443244No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA9680.5047766050644529No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA9540.49747611697467775No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA8830.4604522130908181No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC8460.44115806599641233No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA8210.4281214801218139No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC7830.40830586959242415No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCAAAC7690.401005381502649No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA7580.3952692837178257No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG7320.3817112344082433No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA5940.30974928038045973No Hit
AACTAATCTAAACCTCCTAACCTCAAGCGATCTATCTACTTCTACCTCCG5940.30974928038045973No Hit
ATATAATACCCACCTTCCAAACCACGAACCCACAACCCAATCCTAAAAAC5870.3060990363355721No Hit
TAATACCAACTATACCACTCAACTCCTAAAAATTACCTTCCAAAAACTCT5060.26386049810187306No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACAAAACCCCGAAAAA4940.2576029368820658No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT4750.24769513161737097No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTA4430.23100830169788494No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA4000.20858537399357557No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT3780.1971131784239289No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT3780.1971131784239289No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCAAACACTA3780.1971131784239289No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA3670.19137708063910558No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC3630.18929122689916983No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC3580.18668390972425014No Hit
ATATCTCCTTACATCCCAACCTCATCCATACAACAAAAAACCAAAAACTT3520.1835551291143465No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT3520.1835551291143465No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA3390.1767761044595553No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCAA3230.16843268949981227No Hit
ATATAATACCCACCTTCCAAACCACGAACCCTCAACCCAATCCTAAAAAC2960.15435317675524593No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC2930.15278878645029412No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA2720.1418380543156314No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCAAACC2540.1324517124859205No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA2500.13036585874598472No Hit
AACCCAATCTAAAACCAAAAACCTACTCATCCAATATAAAAAAAACAACT2490.12984439531100078No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAACTCAAACCATAACGAAAAATA2470.1288014684410329No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCTCCACAACCCGAACACTA2450.12775854157106503No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA2360.1230653706562096No Hit
CACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA2360.1230653706562096No Hit
AACAACAATATTTCCTAATCCTCCCAAATAAAAAAAAAAACTCCCCGACA2300.11993659004630595No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAATATTTCGATT2300.11993659004630595No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC2290.119415126611322No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC2220.11576488256643444No Hit
CCTAAAACAAACTAATACCACCCACTAAACTAAAACGAAACCCCGAAAAA2180.11367902882649869No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCGAACACTA2130.11107171165157899No Hit
ACTAAATAAAAACTTACCCAACCCCTCTCTACCAATCCCCTTCCTCCCAT2130.11107171165157899No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC2120.11055024821659505No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2040.10637854073672355No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA2030.1058570773017396No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT2020.10533561386675566No Hit
CTATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT2010.10481415043177172No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACTAAACCCCGAAAAA1970.10272829669183596No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA1960.10220683325685204No Hit
CAAAACTAACTCTAAAATTTCCTTTTTCAATCTTAACCTAATAACCAACT1960.10220683325685204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)