FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000888053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110695
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG60775.489859523917069No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA43773.9541081349654457No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA36413.28921812186639No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT29012.6207145760874475No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA28922.6125841275577035No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA23862.1554722435521025No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC22792.0588102443651475No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT20961.8934911242603552No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG19401.7525633497447943No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT18711.690229911016758No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT17981.624282939608835No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT17931.6197660237589773No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC17851.612538958399205No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA17551.5854374633000587No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC17241.5574325850309407No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA16991.5348480057816523No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA15361.3875965490762907No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG15071.361398437147116No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA14981.353267988617372No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA14511.3108089796287095No Hit
CTCCCAACCCCCACTCCTAAACACACCGTTCCAAAACATATCCTAAACCA13851.2511856904105878No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT13791.2457653913907585No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA13761.2430552418808436No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT13611.2295044943312705No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA12631.1409729436740594No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT12321.1129680654049414No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC11651.052441393016848No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT10980.9919147206287547No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT10340.9340981977505759No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCAAAA9680.874474908532454No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA8300.749808031076381No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA7430.6712136952888568No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC7420.6703103121188853No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCGAAA7260.6558561813993404No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAACTCAAACCATAACGAAAAATA6920.625141153620308No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAGCAAAACGTCTAAAA6370.5754550792718731No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA6310.5700347802520439No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA5940.5366096029630968No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC5810.5248656217534667No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA5710.5158317900537512No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA5530.4995708929942635No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC5410.488730294954605No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA5300.47879308008491805No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCAAACACTA4900.44265775328605633No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG4810.4345273047563124No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC4800.43362392158634083No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA4640.4191697908667962No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCAAACACTA4580.41374949184696685No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA4500.40652242648719455No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC4420.3992953611274222No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT3880.35051266994895885No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCGAAA3830.3459957540991011No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA3530.3188942589999548No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT3330.300826595600524No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT2850.2574642034418899No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA2770.2502371380821175No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCGAACC2730.24662360540223136No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA2720.2457202222322598No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCAAA2620.2366863905325444No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT2600.23487962419260128No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT2560.23126609151271513No Hit
TAATACCAACTATACCACTCAACTCCTAAAAATTACCTTCCAAAAACTCT2510.2267491756628574No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA2430.21952211030308505No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT2420.2186187271331135No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC2400.21681196079317042No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCGAAA2320.2095848954333981No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA2280.20597136275351188No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA2180.19693753105379644No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC2120.1915172320339672No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACCTAAATCCTCC2060.18609693301413793No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCGA2030.18338678350422333No Hit
ATATAATACCCACCTTCCAAACCACGAACCCTCAACCCAATCCTAAAAAC1970.17796648448439406No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA1930.17435295180450788No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT1930.17435295180450788No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCGATCCCCTTCCTCCCAT1670.15086498938524776No Hit
CCTAAAACAAACTAATACCACCCACTAAACTAAAACGAAACCCTAAAAAA1580.14273454085550386No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCAAAC1580.14273454085550386No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC1560.14092777451556077No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACAAAACCCTAAAAAA1550.14002439134558922No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC1540.13912100817561768No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA1450.1309905596458738No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACTAAACCCTAAAAAA1400.12647364379601608No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCTAAACCCATTCGAA1380.12466687745607298No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCTAA1340.12105334477618682No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT1330.12014996160621529No Hit
CTATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT1320.11924657843624374No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT1300.11743981209630064No Hit
CAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT1270.11472966258638602No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA1230.11111612990649986No Hit
AACTAATCTAAACCTCCTAACCTCAAACTATCTATCTACTTCTACCTCCG1210.10930936356655675No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA1210.10930936356655675No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC1180.10659921405664213No Hit
CCCTTTAAACTATCCCACCTTTCTAAACAAAACCAATATACATCTTAAAT1150.10388906454672749No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT1140.10298568137675594No Hit
CAAAACTAACTCTAAAATTTCCTTTTTCAATCTTAACCTAATAACCAACT1130.10208229820678441No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA1120.10117891503681287No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)