FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180800
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGTGGTGG71163.935840707964602No Hit
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGACGGTGAGG70473.8976769911504423No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAG58133.2151548672566372No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTCGTTGACGGTTTT53502.959070796460177No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTACGTTTTGTTGT49202.7212389380530975No Hit
GTTTGGAGGAGTTGGTTTTTTAGAGTTGGTGTTGTTGGTGTTGTATTTTG47482.6261061946902657No Hit
TTGTGGGGATTTAGGTTGTATTATGCGGATGAGGTTTTTAGGTAGTAGGT47142.607300884955752No Hit
GGTAGGAGGGGTTGTATAAGGTTTATTTGGAAGTTAGAATTTATTTGTGG44212.4452433628318584No Hit
GAAGTTGTGGAATTTAGGTAGGAAGTGGTTGATTTTTAGTGTTTAGTTGT43592.410951327433628No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT40752.253871681415929No Hit
TGGGGAGGGAAGAGTAGATTTTACGATATTTTTGGGGTTTTTAGATAGTT40752.253871681415929No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTTAAAGAG38922.1526548672566372No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTTATAGTAGATTGGAGGA38202.1128318584070795No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTCGGAAGGGAG36572.0226769911504427No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTTGGTTTTTTTTGTTTGTTTGT36222.0033185840707963No Hit
TAGAAAGGTGGGATAGTTTAAAGGGGGATTTTTAGGTTATAGGTAAATTT36172.000553097345133No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGCG35701.9745575221238938No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTTAGGGTTTCGTTTTCGTTTAG34551.9109513274336285No Hit
AAAGAGATTTATTTTGAGTGATTATGGTTCGTGATATAGTTTTAGGAGAT32961.823008849557522No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTA28111.5547566371681416No Hit
GGTTTGGAAGGTGGGTATTATATTTTGTTTTGTTTTTGAAGTTTAGTTTT25451.407632743362832No Hit
TTGAGGTTATTAGAAGGGAGGTTTTAGTTATATAGGGTGGAAGAATTTAA23611.3058628318584071No Hit
GTTGGGATTTTAGTTTATTTTTGTAATTTAAATATTTTTTAGTAAAGTTT23431.2959070796460177No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTC23201.2831858407079646No Hit
AAAATATTAGAGGAAGGAGGGGTTTGGGTTTCGTTGGTTTTGTTTAAAAA21181.1714601769911503No Hit
TGTTTGTTTTGGGATTTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTA19721.0907079646017699No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTTGAGGAAATTAAG19311.0680309734513274No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTACGGAGGAGAGGGGTTT19061.0542035398230087No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTCGGGGTTTCGTTTTCGTTTAG17440.9646017699115044No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTTAT16640.9203539823008848No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGTG13910.7693584070796461No Hit
AAAATATTAGAGGAAGGAGGGGTTTGGGTTTTGTTGGTTTTGTTTAAAAA13730.7594026548672566No Hit
GTTTGGAGGAGTTGGTTTTTTAGAGTTGGCGTTGTTGGTGTTGTATTTTG12480.6902654867256638No Hit
GGTTTGGAAGGTGGGTATTATATTTTGTTTTGTTTTCGAAGTTTAGTTTT12290.6797566371681416No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTT10570.5846238938053097No Hit
ATTATTTTTTTAGGGAGTTGGGTTGTATTTTATTTGTTGTTTTGTTTTTA10360.5730088495575221No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTCGGTTTTTTTTGTTTGTTTGT10330.5713495575221239No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTTGGGTGTAGGGGATAA9940.5497787610619469No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGT9740.538716814159292No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTGGGTGTAGGGGATAAG9440.5221238938053098No Hit
GGTTTGGAAGGTGGGTATTATATTTTGTTTTGTTCTCGAAGTTTAGTTTT9040.5No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGCG8040.4446902654867257No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTATAGTAGATTGGAGGAG8020.4435840707964602No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGGGGTAAGCGAGAGGGGGTGTGGT7340.4059734513274336No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTATT7170.39657079646017696No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTATTT6600.36504424778761063No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGCG6240.3451327433628319No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTTA6140.3396017699115044No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTT6080.33628318584070793No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTTGTTGATGGTTTT5410.29922566371681414No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATTGTCGTTTTTTTTTA5140.2842920353982301No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATTGTTGTTTTTTTTTA4450.2461283185840708No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTAG4310.23838495575221239No Hit
GAGAGTAGTAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGTAGG3980.22013274336283184No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTTAT3960.21902654867256635No Hit
TGGGTTTTAGATATTTGTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAGG3880.21460176991150443No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTGGGTGTAGGGGATAAGA3760.2079646017699115No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTTGGAGGTGTTACGTTTTGTTGT3640.2013274336283186No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.1924778761061947No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTTGTTGACGGTTTT3200.17699115044247787No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTTGGTTTTTTTGTTTGTTTGTG3150.17422566371681417No Hit
GTTGTGGTTATTGGTTTTGTGTCGTTTTTTTTTTTGGGCGTAGGGGATAA3140.1736725663716814No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTTAT3130.17311946902654868No Hit
GAAGTTGTGGAATTTAGGTAGGAGTGGGGGTTGGGAGAGACCAAGTCTCT3040.16814159292035397No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGTATTTAATGTTATTATTTAGACAA2830.15652654867256638No Hit
TGTTTGTTTTGGGATTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTAT2770.1532079646017699No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTTTAGGGTTTCGTTTTCGTTTA2740.15154867256637167No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGCG2720.1504424778761062No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTAGGGTTTCGTTTTCGTTTAGT2710.14988938053097345No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTTGGAAGGGAG2660.14712389380530974No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTTTGGGTGTAGGGGATA2540.1404867256637168No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTTTA2500.13827433628318583No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTGAGGAAATTAAGG2440.13495575221238937No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGTG2400.1327433628318584No Hit
GTTGTGGTTATTGGTTTTGTGTCGTTTTTTTTTTGGGCGTAGGGGATAAG2300.12721238938053098No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGTG2090.11559734513274336No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGGATTTTTTTTTTAT2030.1122787610619469No Hit
GAAGTTGTGGAATTTAGGTAGGAAGTGGTTGATTTCTAGTGTTTAGTTGT2030.1122787610619469No Hit
GAGAGTAGTAGGAGGAGGAGGAGGGGGTAAGCGAGAGGGGGTGTGGTAGG1970.10896017699115045No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTTTT1840.10176991150442477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)