FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180800
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG63433.5082964601769913No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA61923.4247787610619467No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG49532.7394911504424777No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA49422.733407079646018No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT47392.621128318584071No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT44092.4386061946902653No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC43482.4048672566371683No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA41232.2804203539823007No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA41022.268805309734513No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA39362.1769911504424777No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT38462.127212389380531No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA37012.047013274336283No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG35211.9474557522123892No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT34201.891592920353982No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT31921.7654867256637168No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT30921.7101769911504423No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC30451.6841814159292035No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT29291.6200221238938055No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA27351.512721238938053No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT27241.5066371681415929No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA25391.4043141592920354No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA24801.3716814159292035No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC23281.2876106194690267No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA22761.258849557522124No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC21971.2151548672566372No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA21181.1714601769911503No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC19591.0835176991150444No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA19361.0707964601769913No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA16880.9336283185840708No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA16190.8954646017699115No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA14960.8274336283185841No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA14280.7898230088495575No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA12350.683075221238938No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA10910.6034292035398231No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA9790.5414823008849557No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA9740.538716814159292No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA9570.5293141592920354No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA9500.5254424778761062No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC8690.480641592920354No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT8680.48008849557522126No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC8630.47732300884955753No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG7570.4186946902654867No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCAAAAAA7310.40431415929203546No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCAAA6970.3855088495575221No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCAAA6790.37555309734513276No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA6310.34900442477876104No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT6170.34126106194690264No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCA4990.2759955752212389No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA4930.27267699115044247No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT4830.26714601769911506No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT4300.23783185840707965No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA4270.2361725663716814No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC4170.23064159292035397No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA4040.22345132743362833No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCAAAC4030.22289823008849557No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCGA4000.22123893805309736No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT3880.21460176991150443No Hit
AAATAATAACATTAAATACTCCTCCCAAACTTGTCTAAATAATAACATTA3880.21460176991150443No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT3720.20575221238938055No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3430.18971238938053098No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTGAACTACCTACCG3410.1886061946902655No Hit
ATATAATACCCACCTTCCAAACCACGAACCCTCAACCCAATCCTAAAAAC3400.18805309734513273No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA3390.1875No Hit
ATATAATACCCACCTTCCAAACCACGGACCCGCAACCCAATCCTAAAAAC3380.18694690265486727No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC3340.18473451327433627No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT3140.1736725663716814No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAGACTTCTCCATCTT3140.1736725663716814No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC2970.1642699115044248No Hit
AAATAATAACATTAAATACTCCTCCCAAATACTCAAACTAAAACCCTTAA2960.16371681415929204No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCGAACC2950.16316371681415928No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2840.1570796460176991No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA2810.1554203539823009No Hit
CCCAAAATCCTAAAATTCTCCTAACCTCAAAAACTCCCCACAAAAACAAA2690.14878318584070796No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCTCCACGACCCGAACACTA2570.14214601769911506No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC2550.14103982300884957No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAG2520.13938053097345132No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA2380.1316371681415929No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCTAAAACTTCTCCATCTT2330.1288716814159292No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC2300.12721238938053098No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTA2250.12444690265486726No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT2250.12444690265486726No Hit
AAAAACTAACACACACTTATACCCCCTAAACAACCATAAATACACAAAAA2230.12334070796460175No Hit
CTATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT2120.1172566371681416No Hit
TCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG2080.1150442477876106No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAGC2050.11338495575221239No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAACATAAAAAAAACAACT2040.11283185840707964No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA1900.10508849557522124No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)