FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000888065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44577
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA15703.5219956479799004No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA12022.696457814568051No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG11912.671781411938892No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA10272.3038786818314376No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC9982.238822711263656No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA9962.2343360926038094No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA9162.054871346209929No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA8701.9516791170334478No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT8611.931489333064136No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA8411.8866231464656662No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT8231.846243578527043No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC8071.8103506292482672No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC8041.8036207012584966No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT7851.7609978239899502No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT7671.7206182560513268No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA7481.6779953787827804No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT7381.6555622854835454No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC7041.5792897682661462No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT6801.525450344347982No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA6621.4850707764093591No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT6571.4738542297597417No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG6031.3527155259438726No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC5871.3168225766650963No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT5801.301119411355632No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA5181.1620342329003748No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA4501.0094891984655765No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT4450.9982726518159589No Hit
CCCTTTAAACTATCCCACCTTTCTAAACAAAACCAATATACATCTTAAAT4000.8973237319694013No Hit
AACTAATCTAAACCTCCTAACCTCAAGCGATCTATCTACTTCTACCTCCG3740.8389976893913902No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG3620.8120779774323081No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA3580.803104740112614No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA3250.7290755322251385No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA2870.6438297776880454No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCAAACC2840.6370998496982749No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC2780.6236399937187339No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT2720.6101801377391929No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA2690.6034502097494223No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA2440.5473674765013348No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA2400.5383942391816408No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC2220.4980146712430177No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA2150.48231150593355315No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC2090.46885164995401213No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCGAACACTA2060.46212172196424167No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCAAA1990.44641855665477714No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA1970.4419319379949301No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA1940.4352020100051596No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACAAAACCCTAAAAAA1870.4194988446956951No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA1860.41725553536577153No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA1620.36341611144760755No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA1600.3589294927877605No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCGAAA1540.34546963680821946No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACCAACCCATCTCCGA1520.34098301814837245No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT1510.33873970881844895No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC1330.29836014087982593No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCAAA1320.2961168315499024No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACCAACCCATCTCCGAG1150.25798057294120286No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA1130.25349395428135585No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC1060.23779078897189135No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT1060.23779078897189135No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCAAAC1020.2288175516521973No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG1010.22657424232227383No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACAAAACACCCAAAA990.2220876236624268No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA950.21311438634273283No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAGAGTTTCGGTT940.2108710770128093No Hit
AACTAATCTAAACCTCCTAACCTCAATCGATCTATCTACTTCTACCTCCG920.20638445835296232No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA910.2041411490230388No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCGATCCCCTTCCTCCCAT900.20189783969311528No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACCACAAAACACCCAAAA890.1996545303631918No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT870.19516791170334477No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT840.18843798371357426No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC830.18619467438365075No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA800.17946474639388024No Hit
ATATAATACCCACCTTCCAAACCACGAACCCTCAACCCAATCCTAAAAAC800.17946474639388024No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA790.17722143706395674No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCGAACC770.17273481840410973No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT770.17273481840410973No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT770.17273481840410973No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA760.17049150907418623No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT750.16824819974426275No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA730.1637615810844157No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT730.1637615810844157No Hit
TACCTTCCAAAAACTCTAAACATCCCCACACAACTACAAAACACCCAAAA720.16151827175449224No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCGA700.15703165309464523No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACTAAACCCTAAAAAA680.15254503443479822No Hit
CCTAAAACAAACTAATACCACCCACTAAACTAAAACGAAACCCTAAAAAA640.1435717971151042No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC640.1435717971151042No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC620.1390851784552572No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCCACTAACCCATCTCCGAA610.1368418691253337No Hit
CTATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT580.13011194113556318No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA570.12786863180563968No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCAA560.12562532247571617No Hit
CCAAAACTCAACAACCTAAACCCCAAAAATAACTACTAAACTACCTACCG560.12562532247571617No Hit
AACTAATCTAAACCTCCTAACCTCAAATAATCCACCTACCTCAACCTCCC560.12562532247571617No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAACCCCCCCAACAA560.12562532247571617No Hit
TAATACCAACTATACCACTCAACTCCTAAAAATTACCTTCCAAAAACTCT540.12113870381586916No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCCACTAACCCATCTCCGA540.12113870381586916No Hit
CAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC510.11440877582609865No Hit
AAAAACTAACACACACTTATACCCCCTAAACAACCATAAATACACAAAAA510.11440877582609865No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAATTCCACTAACCCCAAACCC510.11440877582609865No Hit
CCCAAAATCCTAAAATTCTCCTAACCTCAAAAACTCCCCACAAAAACAAA510.11440877582609865No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA510.11440877582609865No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC490.10992215716625166No Hit
CCAAAACTCAACAACCTAAACCCCGAGAATAACTACTAAACTACCTACCG480.10767884783632814No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCTAAAACTTCTCCATCTT480.10767884783632814No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT450.10094891984655764No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)