FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238773
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA81953.4321300984617187No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG80203.358838729672115No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT62442.6150360384130535No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA57762.4190339778785708No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT57592.411914244910438No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT54482.281665012375771No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC53152.2259635720956723No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG51932.1748690178537777No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT51162.1426208155863518No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA50812.127962541828431No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA49172.059278059077031No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA47061.9709096087078524No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG44971.8833787739819827No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC44761.8745838097272305No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC41281.728838687791333No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA39771.6655987067214468No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT37241.5596403278427629No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT35621.4917934607346728No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT35581.490118229448053No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA34691.452844333320769No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA32861.3762025019579265No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC32781.3728520393846877No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT30411.2735945856524817No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT28401.1894142134998513No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA25701.0763361016530344No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA24671.0331988960225822No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA24471.0248227395894844No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA23760.9950873842519883No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA23660.9908993060354395No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA23450.9821043417806871No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC22500.9423175987234738No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA21410.8966675461630922No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA18830.7886151281761338No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA15970.6688360911828389No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA15670.6562718565331925No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC14610.6118782274377756No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA14440.6047584944696427No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC14110.5909378363550317No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCCAAAA14060.5888437972467574No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC13900.5821428721002793No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA13670.5725102922022172No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAGATA11120.4657142976802235No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA10960.45901337253374547No Hit
ATATAATACCCACCTTCCAAACCACAAACCCACAACCCAATCCTAAAAAC9740.4079188182918504No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG8740.3660380361263627No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA8500.3559866484066456No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA8410.35221737801175174No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCGAAA7780.3258324852474945No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT7720.3233196383175652No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCGA7160.2998664003048921No Hit
AACCCAATCTAAAACCAAAAACCTATCTCCACATTAATATCATTAACAAC7090.29693474555330795No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT6910.2893962047635202No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT6570.27515673882725433No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCAAA6500.2722250840756702No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA6470.27096866061070557No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCAAACACTA6280.2630113119992629No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAGCAAAACGTCTAAAA6180.25882323378271416No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACAAAACACCCAAAA6130.2567291946744398No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA6000.25128469299292633No Hit
TAATACCAACTATACCACTCAACTCCTAAAAATTACCTTCCAAAAACTCT5890.24667780695472266No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC5760.2412333052732093No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT5710.23913926616493492No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA5590.23411357230507637No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT5480.22950668626687273No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA5260.2202929141904654No Hit
CCCAAAATCCTAAAATTCTCCTAACCTCAAAAACTCCCCACAAAAACAAA5100.21359198904398738No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC4730.19809609964275693No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCTAAAAAA4700.19683967617779233No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCGAACC4680.19600206053448252No Hit
AACTAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCG4620.1934892136045533No Hit
ATATAATACCCACCTTCCAAACCACGAACCCTCAACCCAATCCTAAAAAC4460.18678828845807524No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT4450.18636948063642036No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC4280.17924974766828744No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAACCTTACACACCCCCAAAATTA4210.1763180929167033No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT4170.1746428616300838No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTGCTAACCCATCTCCGA4050.16961716777022529No Hit
AACTAATCTAAACCTCCTAACCTCAAGCGATCTATCTACTTCTACCTCCA3920.16417266608871187No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT3870.16207862698043748No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCGAACACTA3720.1557965096556143No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCTAAAACTTCTCCATCTT3540.14825796886582654No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3490.14616392975755216No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT3340.139881812432729No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT3120.13066804035632168No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3100.12983042471301195No Hit
CAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC2950.1235483073881888No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCAAAC2930.12271069174487903No Hit
CAAAACTAACTCTAAAATTTCCTTTTTCAATCTTAACCTAATAACCAACT2920.12229188392322415No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCGATCCCCTTCCTCCCAT2900.12145426827991439No Hit
CTATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT2900.12145426827991439No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA2890.12103546045825953No Hit
CACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG2830.11852261352833025No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAACCCCCCCAACAA2830.11852261352833025No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC2820.11810380570667538No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT2800.11726619006336561No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCTAAACCCATTCGAA2790.11684738224171075No Hit
AAAAACTAACACACACTTATACCCCCTAAACAACCATAAATACACAAAAA2740.11475334313343637No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTGCTAACCCATCTCCGAA2730.11433453531178148No Hit
ATATCTCCTTACATCCCAACCTCGTCTATACAACAAAAAACCAAAAACTT2570.10763361016530346No Hit
AACTAATCTAAACCTCCTAACCTCAAACTATCTATCTACTTCTACCTCCG2440.10218910848379004No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCTCCACAACCCGAACACTA2440.10218910848379004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)