FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000888072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109534
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGTGGTGG79047.216024248178647No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTTAGGGTTTCGTTTTCGTTTAG59295.412931144667409No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTA57495.248598608651195No Hit
GTTTGGAGGAGTTGGTTTTTTAGAGTTGGTGTTGTTGGTGTTGTATTTTG54764.9993609290266035No Hit
TTGTGGGGATTTAGGTTGTATTATGCGGATGAGGTTTTTAGGTAGTAGGT50464.6067887596545365No Hit
GGTAGGAGGGGTTGTATAAGGTTTATTTGGAAGTTGGAATTTATTTGTGG48904.4643672284404845No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTTAAAGAG47804.363941789763909No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTCGGAAGGGAG43423.964065952124454No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTC41493.7878649551737356No Hit
TAGAAAGGTGGGATAGTTTAAAGGGGGATTTTTAGGTTATAGGTAAATTT35923.2793470520568953No Hit
AAAATATTAGAGGAAGGAGGGGTTTGGGTTTTGTTGGTTTTGTTTAAAAA29542.696879507732759No Hit
TTGAGGTTATTAGAAGGGAGGTTTTAGTTATATAGGGTGGAAGAATTTAA26142.386473606368799No Hit
GAGAGTAGTAGGAGGAGGAGGAGGGGGTAAGCGAGAGGGGGTGCGGTAGG24222.2111855679515036No Hit
GGTTAGGAGAATTTTAGGATTTTGGGAGGTTTAGGCGGGAGGATTAGGAA19591.788485766976464No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTACGGAGGAGAGGGGTTT19271.7592710939069147No Hit
GAGAGTAGTAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGTAGG18091.6515419869629522No Hit
TGTTTGTTTTGGGATTTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTA15771.4397356072087206No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTTGAGTTTGGTTTTTTTTTATT14841.3548304636003432No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGCG14031.280880822393047No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTT12991.1859331349170121No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTTAT12731.1621962130480035No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTAG9420.860006938484854No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTTAT9370.8554421458177369No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGTG9090.8298793068818814No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTCGGGTGTAGGGGATAA7660.6993262366023335No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTTGAGGAAATTAAG7320.6682856464659376No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTTGGAAGGGAG7140.6518523928643161No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTACTTTTTTAAAGAG5300.48386802271440826No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTATT5160.47108660324648055No Hit
TAGAAAGGTGGGATAGTTTAAAGGGGGGTTTTTAGGTTATAGGTAAATTT5150.4701736447130571No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTAGGGTTTCGTTTTCGTTTAGT4920.44917559844431865No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTATGGAGGAGAGGGGTTT4640.4236127595084631No Hit
GGTAGTTGGGTGATTTTTTTATAGGATTTTTAGGGAGTGGTTGGAGACCA4430.40444063030657146No Hit
AAAATATTAGAGGAAGGAGGGGTTTAGTGGGTGAGTTGTTTTTTTTTTAG4110.3752259572370223No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTATT3600.3286650720324283No Hit
GAAGTTGTGGAATTTAGGTAGGAGTGGGGGTTGGGAGAGACCAAGTCTCT3590.3277521134990049No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTCGGGTGTAGGGGATAAG3450.3149706940310771No Hit
TGTTTGTTTTGGGATTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTAT3190.29123377216206836No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTTGAGTTTGGTTTTTTTTATTT2340.2136322968210784No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.1926342505523399No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTTTA2050.18715649935179945No Hit
GAGAGTAGTAGGAGGAGGAGGGGGTAAGCGAGAGGGGGTGCGGTAGGAGT1980.18076578961783554No Hit
GGAAGGTATGTGGGGTTTAGTTTTAGTTTGGTATCGTCGTTTTTTTTTAG1720.15702886774882685No Hit
AAAATATTAGAGGAAGGAGGGGTTTAGTGGGTGAGTTGTTTTTTTTTAGA1640.14972519948143956No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTTTT1570.14333448974747567No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTTCGGGTGTAGGGGATA1530.13968265561378201No Hit
AAAATATTAGAGGAAGGAGAGGTTGGGATGTAAGGAGATATAGACCAAGT1480.13511786294666495No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTTTA1400.12781419467927768No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTTTAGGGTTTCGTTTTCGTTTA1310.11959756787846697No Hit
GGGGATTGGGATAAAGGAGTATTATCTTCGGTTTTTGTTTGAGTGATTTC1300.11868460934504355No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTTGAGTTTGGTTTTTTTTTTAT1260.11503277521134989No Hit
TGTTTGTTTTGGGATTTTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGT1230.11229389961107966No Hit
GAGAGTAGTAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGTAGGAGT1220.11138094107765627No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTACGGTGGAGAGGGGTTT1130.10316431427684555No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTAAAGAGT1120.10225135574342212No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)