FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000888073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109534
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA74796.828016871473698No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC58545.344459254660653No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACATCCTAAAACA52654.806726678474264No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC46594.253473807219676No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT45054.112878193072471No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA44184.033450800664634No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCAAACACTA42753.902897730385086No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA42393.8700312231818432No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG39073.5669289900852705No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA34803.1770956963134735No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC33573.0648017967023935No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCAAA26882.4540325378421315No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT26362.4065586941041137No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC24762.260485328756368No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA23252.1226285902094326No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT21881.9975532711304251No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT18581.6962769551006993No Hit
AACAACAATATTTCCTAATCCTCCCGCCTAAACCTCCCAAAATCCTAAAA17281.577592345755656No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA15221.3895228878704329No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA13861.265360527324849No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG9750.8901345700878267No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCGATCCCCTTCCTCCCAT9640.8800920262201691No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA7350.6710245220662078No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC6580.600726714992605No Hit
ATATCTCCTTACATCCCAACCTCCTCCCCAAAACAAACGAAAAAATACCT6290.5742509175233261No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT5940.5422973688535067No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT5460.4984753592491829No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCTCCACGACCCAAACACTA5290.48295506418098494No Hit
ATATAATACCCACCTTCCAAACCACGAACCCTCAACCCAATCCTAAAAAC5260.48021618858071463No Hit
CCAACCACTCCCTAAAAATCCTATAAAAAAATCACCCAACTACCTGTAGA4710.430003469242427No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA4690.4281775521755802No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT4300.3925721693720671No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC4220.3852685011046798No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC3710.3387076159000858No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT3700.33779465736666237No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC3660.3341428232329688No Hit
CCTAAAACAAACTAATACCACCCACTAAACTAAAACGAAACCCTAAAAAA3630.33140394763269854No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCAAACACTA3580.32683915496558147No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3530.3222743622984644No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACTAAACCCTAAAAAA3510.3204484452316176No Hit
CAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC3230.29488560629576205No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCTAAACCCATTCAAA3100.2830171453612577No Hit
AACTAATCTAAACCTCCTAACCTCAAACTATCTATCTACTTCTACCTCCG2610.2382821772235105No Hit
CAAAACTAACTCTAAAATTTCCTTTTTCAATCTTAACCTAATAACCAACT2540.23189146748954664No Hit
ATATAATACCCACCTTCCAAACCACTAACCCTCAACCCAATCCTAAAAAC2450.22367484068873592No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2410.22002300655504228No Hit
CACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG2100.1917212920189165No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCTAA2040.18624354081837602No Hit
CTATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT2030.1853305822849526No Hit
AACAACAATATTTCCTAATCCTCCCTCCTAAACCTCCCAAAATCCTAAAA1920.1752880384172951No Hit
CCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC1700.15520295068198003No Hit
AACCCAATCTAAAACCAAAAACCTACTCATCCAATATAAAAAAAACAACT1560.14242153121405227No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACATCCTAAAGCA1560.14242153121405227No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACTAAAAATA1530.13968265561378201No Hit
CACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC1430.1305530702795479No Hit
ATATCTCCTTACATCCCAACCTCTCCTTCCTCTAATATTTTTGTAGAACC1420.1296401117461245No Hit
ATCAAAAAACCCCCAACCACAACCGAAACCCTCCTCCCCTACCCCGACCT1400.12781419467927768No Hit
ACTAAATAAAAACTTACCCAACCCCTCTCTACCGATCCCCTTCCTCCCAT1290.11777165081162014No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAGAAC1250.11411981667792648No Hit
TAAAACTCACTACTATCCCCCACAAATACACCTAAAACTCACTACTATCC1220.11138094107765627No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAAACTCTACTAACCCATCTC1220.11138094107765627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)