FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215530
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG98854.58636848698557No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA78993.664919036793022No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG78143.625481371502807No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA71903.3359625110193476No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA67443.1290307613789268No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT66943.1058321347376237No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT65733.049691458265671No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT48832.265577877789635No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT47032.182062821880945No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA46392.152368579780077No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC45802.1249942003433397No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA39361.8261958892033592No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA39181.81784438361249No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA38931.8062450702918387No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC37911.758919871943581No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT35301.6378230408759802No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT34631.6067368811766343No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA34431.5974574305201132No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT31661.4689370389272955No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA28851.3385607572031735No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCCACCCACCTTAACTTCCC26791.2429824154410059No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA25971.2049366677492692No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA22391.038834500997541No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC22201.030019022873846No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT21691.006356423699717No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG21601.0021806709042824No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA18570.8615969934579872No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC18410.8541734329327705No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC18400.8537094603999442No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC18180.8435020646777711No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA17930.8319027513571198No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC16650.7725142671553844No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCCAAAA15900.7377163271934302No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA14800.6866793485825639No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA14560.6755440077947386No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC13640.6328585347747414No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA13180.6115157982647428No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA12790.5934208694845265No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAGACTCT12510.580429638565397No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCGAACACTA12330.5720781329745279No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC11610.5386721106110518No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA11480.5326404676843131No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT11180.5187212916995314No Hit
ATATAATACCCACCTTCCAAACCACGAACCCACAACCCAATCCTAAAAAC10730.4978425277223589No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA9200.4268547301999721No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAACA8950.41525541687932077No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC8740.40551199368997354No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT7560.35076323481649885No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA7090.3289565257736742No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG7090.3289565257736742No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT5890.2732798218345474No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA5850.2714239317032432No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCGA5700.2644643437108523No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT5630.2612165359810699No Hit
ATATAATACCCACCTTCCAAACCACCATCCTTTTCTCCTTAAAACTAAAC5410.2510091402588967No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCATCTCCTAAAACA5280.24497749733215793No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCGAACC5260.24404955226650585No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA5050.23430612907715864No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCA4860.22549065095346355No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA4790.22224284322368118No Hit
CCCTTTAAACTATCCCACCTTTCTAAACAAAACCAATATACATCTTAAAT4700.21806709042824665No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT4630.21481928269846423No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT4570.2120354475015079No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCTCCACGACCCGAACACTA4400.20414791444346492No Hit
ATATAATACCCACCTTCCAAACCACAAACCCTCAACCCAATCCTAAAAAC3950.18326915046629239No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA3370.1563587435623811No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3200.14847121050433815No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCTAAAACTTCTCCATCTT3150.14615134784020786No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCAATT2980.13826381478216487No Hit
CCCAAAATCCTAAAATTCTCCTAACCTCAAAAACTCCCCACAAAAACAAA2920.13547997958520855No Hit
CCTAAAACAAACTAATACCACCCACTAAACTAAAACGAAACCCTAAAAAA2900.13455203451955644No Hit
CAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT2810.13037628172412194No Hit
CTATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC2770.1285203915928177No Hit
CACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA2760.12805641905999166No Hit
CAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC2680.1243446387973832No Hit
AAAAACTAACACACACTTATACCCCCTAAACAACCATAAATACACAAAAA2660.12341669373173109No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT2650.12295272119890502No Hit
CCAAAACTCAACAACCTAAACCCGAAAATAACTACTAAACTACCTACCGC2600.12063285853477475No Hit
CAAAACTAACTCTAAAATTTCCTTTTTCAATCTTAACCTAATAACCAACT2450.11367327054238388No Hit
CTATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT2440.11320929800955784No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACTAAACCCTAAAAAA2430.11274532547673177No Hit
AACCCAATCTAAAACCAAAAACCTACTCATCCAATATAAAAAAAACAACT2360.10949751774694938No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAACCCCCCCAACAA2360.10949751774694938No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2340.10856957268129727No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAACCTATTACATCTACTCCGA2280.10578573748434093No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC2230.10346587482021065No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)