FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000888079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145810
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA69534.76853439407448No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG67904.6567450792126746No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG59364.071051368218915No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT51703.5457101707701804No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA49003.3605376860297644No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA44223.0327138056374734No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA40452.7741581510184488No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA38672.6520814758932856No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC37522.57321171387422No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC35242.4168438378712023No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT35052.4038131815376174No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG32672.240587065359029No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA32202.2083533365338455No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT30992.125368630409437No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA29071.9936904190384748No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT27741.9024758247033813No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA25821.7707976133324188No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCGAAA24551.6836979631026678No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA23361.6020849050133734No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC21671.4861806460462246No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT20651.4162265962554008No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA20521.4073108840271586No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA17371.1912763184966737No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA15491.06234140319594No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA14931.0239352582127426No Hit
TAAAACTCACTACTATCCCCCACAAAAAACATAAAAAAATTCCAAACTAT14661.005418009738701No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC12050.8264179411562994No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC11950.8195597009807284No Hit
CCAAAACTCAACAACCTAAACCCCAAAAATAACTACTAAACTACCTACCG11940.8188738769631712No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA11490.788011796173102No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA11270.7729236677868458No Hit
CCCAAAATCCTAAAATTCTCCTAACCTCAAAAACTCCCCACAAAAACAAA8820.6048967834853577No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG8370.5740347026952884No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA6490.4450997873945545No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA6480.44441396337699746No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA6480.44441396337699746No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA5800.397777930183115No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCGAACC5590.38337562581441603No Hit
ATATAATACCCACCTTCCAAACCACAAAACCCACCACACCAACCACCTAA5350.3669158493930457No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCGA5310.36417255332281734No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT5310.36417255332281734No Hit
AATCTAAAAACCTCCTCTCCAAAACCTTATACAACCCCTCCTACCTGTAG4820.3305671764625197No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT4510.3093066319182498No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA4050.2777587271106234No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCAAAA4040.2770729030930663No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3670.2516974144434538No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC3340.2290652218640697No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT3220.2208353336533845No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2940.2016322611617859No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA2710.1858583087579727No Hit
CCTAAAACAAACTAATACCACCCACTAAACTAAAACGAAACCCTAAAAAA2640.18105754063507304No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC2590.17762842054728756No Hit
CAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC2540.17419930045950208No Hit
AAAAACTAACACACACTTATACCCCCTAAACAACCATAAATACACAAAAA2500.17145600438927372No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACGACTACAAAACACCCGAAA2480.17008435635415953No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAGTCCCCTTCCTCCCAT2390.16391194019614566No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACTAAACCCTAAAAAA2310.15842534805568892No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAACCTATTACATCTACTCCGA2280.1563678760030176No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAAACCCCCCC2260.15499622796790344No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT2200.15088128386256086No Hit
CAAAACTAACTCTAAAATTTCCTTTTTCAATCTTAACCTAATAACCAACT2160.14813798779233248No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAACCCCCCCAACAA2110.144708867704547No Hit
CTATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT2060.14127974761676154No Hit
CTCCAAACTACAAAAAACTCCTTCAATCACCTATCCACCTCCTAAAACAA2020.13853645154653316No Hit
CACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG1910.13099238735340513No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT1840.12619161923050545No Hit
ATATAATACCCACCTTCCAAACCACACATAAAACTAAACCCCACATACCT1790.12276249914271997No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA1750.1200192030724916No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCTAA1720.1179617310198203No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCTAAA1720.1179617310198203No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA1710.11727590700226322No Hit
CACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCGAAA1700.11659008298470612No Hit
CCAAAAAAACCAACAAAAAAACTAACAAAAAAACCAACAAAAAAACCAAC1700.11659008298470612No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAACA1680.11521843494959193No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCAA1670.11453261093203485No Hit
AACTAATCTAAACCTCCTAACCTCAAACTATCTATCTACTTCTACCTCCG1660.11384678691447774No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAAACCTATTACATCTACTCC1630.11178931486180647No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAATTCCACTAACCCCGAACCC1630.11178931486180647No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAAATTCCACTAACCCCG1590.10904601879157808No Hit
CACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT1550.10630272272134969No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)