FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000888085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21428
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA247211.536307634870264No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT19369.034907597535934No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA15347.158857569535187No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA14126.589509053574762No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT13606.346835915624418No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA8363.9014373716632447No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG5302.473399290647751No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA4992.3287287661004292No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT4822.2493933171551244No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG4302.0067201792047786No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT3741.7453798767967144No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC3731.7407130856822848No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA3601.6800448011946985No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT3421.5960425611349633No Hit
TAATACCAACTATACCACTCAACTCCTAAAAATTACCTTCCAAAAACTCT3361.5680418144483854No Hit
GCTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA3341.5587082322195258No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA3311.5447078588762366No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT2140.9986932984879596No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA1930.9006906850849357No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCGA1920.8960238939705059No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC1590.7420197871943252No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCGAACC1540.7186858316221766No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCTAA1260.5880156804181444No Hit
GAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA1150.5366809781594176No Hit
CAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT870.4060108269553855No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG830.3873436624976666No Hit
GCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT810.37801008026880717No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCTAACACTA730.3406757513533694No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA700.3266753780100803No Hit
AAAAACTAACACACACTTATACCCCCTAAACAACCATAAATACACAAAAA700.3266753780100803No Hit
CCCTTTAAACTATCCCACCTTTCTAAACTAAACCAATATACATCTTAAAT630.2940078402090722No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAAACTCTACTAACCCATCTC610.2846742579802128No Hit
GCTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT570.2660070935224939No Hit
GACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG490.22867276460705618No Hit
ACTAAATAAAAACTTACCCAACCCCTCTCTACCAATCCCCTTCCTCCCAT470.21933918237819675No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCTAA470.21933918237819675No Hit
CAAATCCCTAATCTCACCTAAAAAAAACTCTACTAACCCATCTCCGAATC460.21467239126376705No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAAATTCCACTAACCCCG460.21467239126376705No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC440.20533880903490762No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT420.19600522680604818No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCCAAATCC370.17267127123389958No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACTAAACCCTAAAAAA360.16800448011946986No Hit
CCTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT330.1540041067761807No Hit
GCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT320.14933731566175099No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC320.14933731566175099No Hit
CAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA310.14467052454732127No Hit
GAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA300.14000373343289155No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT290.13533694231846183No Hit
CCTAAAACAAACTAATACCACCCACTAAACTAAAACGAAACCCTAAAAAA290.13533694231846183No Hit
CACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG290.13533694231846183No Hit
AACTAATCTAAACCTCCTAACCTCAAACTATCTATCTACTTCTACCTCCG280.1306701512040321No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC270.1260033600896024No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCAAA270.1260033600896024No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCTAAC260.12133656897517267No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC250.11666977786074295No Hit
ACTACAAACCCTACAACAAATCCATACCCCCACCACAACCCGAACACTAC240.11200298674631323No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACTACCCGAACACTA240.11200298674631323No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAAAATTCCACTAACCCC230.10733619563188353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)