FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307081
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC97193.164962990220821No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT76242.482732568931324No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA68662.2358921587463896No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA66652.170437115940094No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT64262.0926074879266383No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC63672.0733943161576263No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA63632.072091728241083No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA61021.9870978666866397No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG59151.9262018815882453No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT52521.7102979344212115No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC52491.7093209934838038No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA52431.7073671116089892No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT52271.7021567599428165No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC47651.5517078555820776No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC46641.518817510689362No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA46401.511001983190103No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC44371.4448956464255358No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA42411.3810688385149195No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT41801.361204372787636No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC41771.3602274318502285No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC41001.335152614456772No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA40391.3152881487294883No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT40271.3113803849798589No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG40031.3035648574805996No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA38801.2635102790468966No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA37961.2361559327994895No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA34651.1283667827055401No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT33741.0987329076041825No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA33561.0928712619797383No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA31471.0248110433403563No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC30841.0042952836548011No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC28680.9339555361614688No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA28140.9163705992881357No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA26970.8782699027292473No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC26410.8600336718976427No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC25230.8216073283596186No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC24560.79978898075752No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC22210.7232619406606074No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT18790.6118906737961646No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT18550.6040751462969054No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT18200.5926775020271524No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA17730.5773720940077699No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC17560.5718360953624614No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA17110.5571819813013504No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA16540.53862010349061No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC16260.5295019880748076No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA15960.5197325787007336No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC15530.5057297585978944No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA14620.47609588349653675No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC13610.44320553860382117No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT12850.41845636818950044No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT12620.4109664876693771No Hit
CAACTACCAAACCCTAAAAAACCACCTCCCTACACAACACCTCCTACCAC12260.3992431964204884No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACACACACTTCAAT12010.3911020219420935No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC11710.38133261256801954No Hit
CATAATCACCCACAACAAATTTCCCGCCACCGAAATAAGCCACCCCCTAA9860.3210879214278969No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC9710.3162032167408599No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA9640.31392368788690933No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC9080.29568745705530464No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA8490.27647428528629253No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCGCC7690.2504225269554287No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT7650.2491199390388855No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA7450.24260699945616954No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA7100.2312093551864166No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC7000.22795288539505862No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA6720.2188347699792563No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA6500.21167053643826872No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6370.20743712570950334No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA6370.20743712570950334No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG6230.20287806800160219No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC5970.19441124654407144No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC5880.19148042373184926No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC5400.17584936873333096No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAACACTAAA5280.17194160498370137No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA5280.17194160498370137No Hit
CCATAAAAACAAAAACCTAAAAAAAACTCAACAAAACTTCCAAAAACCCC4740.15435666811036827No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAGCCCCCACCACCCGCAA4710.1533797271729609No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC4470.14556419967370174No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC4350.14165643592407215No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT4260.13872561311184997No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA4170.13579479029962777No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA4070.1325383205082698No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT4070.1325383205082698No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT4000.13025879165431922No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCGAAAAAAAAAACGACACA3990.1299331446751834No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC3990.1299331446751834No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCAGCCTCG3810.12407149905073905No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACGCC3710.12081502925938108No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCCGTCCTATAA3670.1195124413428379No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA3660.11918679436370208No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT3560.11593032457234412No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCAATCCACTC3530.1149533836349367No Hit
CACTCCATTATTCTTATCTTTAATCTCTTTTTATTTATAAAAATCCAATT3340.10876609103135655No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)