FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000888089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221414
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT72333.266731101014389No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC59492.6868219715103834No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG55482.5057132791964376No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA48872.207177504584173No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC48072.171046094646228No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT47662.152528747053032No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA47262.13446304208406No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA44051.9894857597080582No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC43471.9632904875030486No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT39891.8016024280307477No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA39521.7848916509344486No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA39511.7844400083102243No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA38671.7465020278753827No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC36561.6512054341640547No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA34491.5577154109496238No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT34171.543262846974446No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT33721.5229389288843522No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA32971.4890657320675296No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC31401.4181578400643138No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC31041.401898705592239No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC30971.3987372072226687No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA29061.3124734659958268No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC28771.299375829893322No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA28431.2840199806696957No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC28071.2677608461976206No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA26831.2117571607938071No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA26031.1756257508558627No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA23711.0708446620358243No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG23001.0387780357158987No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC22341.0089696225170948No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT22241.0044531962748517No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA22080.9972269142872627No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA21450.9687734289611316No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA20630.9317387337747387No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT20400.9213509534175798No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT19280.8707669795044577No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC19260.8698636942560092No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT18180.8210862908397842No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA17140.7741154579204568No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC16960.7659858906844192No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC13390.6047494738363428No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT12410.560488496662361No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC11920.5383580080753702No Hit
AACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTAATTTATAC11620.524808729348641No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC11170.5044848112585474No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC11000.4968068866467341No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC9480.42815720776463995No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCACAAAAC7950.3590558862583215No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACGT7800.35228124689495693No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC7660.3459582501558167No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA7470.33737704029555493No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT6810.30756862709675087No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACACTACCTT6640.2998907024849377No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA6480.29266442049734886No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA6420.28995456475200304No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA6260.28272828276441414No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATGCCTGGAGTGG6200.28001842701906837No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC6130.2768569286494982No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCACTTACCAATCCACTC6110.2759536434010496No Hit
CAAAACAAACAACACTAAAACCAAATAACCTTCTCAAAAAACCCTTTCTG5970.26963064666190933No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC5760.260146151553199No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT5660.2556297253109559No Hit
CAACTACCAAACCCTAAAAAACCACTTACACAAAAAATAACTAAAAACAA5570.2515649416929372No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC5550.25066165644448857No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA5180.23395087934818937No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCGCC5150.23259595147551645No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA4750.21453024650654431No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA4580.20685232189473113No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT4240.1914964726711048No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA4210.1901415447984319No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC4130.18652840380463748No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCAATCCACTC4090.18472183330774025No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4080.18427019068351594No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACGAACGAACC3920.1770439086959271No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA3920.1770439086959271No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATGAACCACCCCCTAA3500.15807491847850633No Hit
AACTTACACATCTCAAAAAAACCCAGGGAACCCCGCAGCCACGCTACCTT3390.153106849612039No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC3240.14633221024867443No Hit
TACCACCTAAATCTCCAACTTCTCCAAAAAAACCAACTATCCTAATAAAA3210.14497728237600152No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG3110.14046085613375847No Hit
AAACCTATCAAAACCTCCCCACACTCCAACCAACCAACAAAAAACACACA3090.13955757088530987No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT3090.13955757088530987No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA3080.1391059282610856No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC2780.12555664953435647No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA2770.12510500691013215No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC2730.12329843641323494No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC2710.12239515116478632No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC2680.12104022329211342No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT2670.1205885806678891No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA2540.11471722655297316No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT2460.11110408555917872No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC2440.11020080031073014No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT2310.10432944619581418No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC2220.10026466257779544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)