FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267311
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGTTGTTTTGGTTTAGAGAGGGCGGGAGTTTTTGTAGGATAATATAGT79462.9725675336967057No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA66722.495969114626783No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAACGGTTT63082.3597981377496624No Hit
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGCGGGTA62792.348949351130331No Hit
GGTGGGGAATAAGGTTTTGTATTTATTGAGTTTTTGTTATAGGTTTGGGT58972.206044644627419No Hit
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAGTATTGAGGTTAGGTTTG58732.1970663384596967No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTT58462.186965744021009No Hit
GATAGGTTTGGAGATAGTTTTGTTTAAAGATATTTAGTATTTTAGTGGTA57312.143944693634007No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGGTTTTTTGT56252.1042905080599006No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATAGATGAGGTTGGGATGTAAGGAGA56062.097182682343787No Hit
GGTTGTAGGAGGTTTTTAGTAAGGGAGGGGTTGTAGGAGTGTTTTTGGGG55422.073240532563194No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGCGGGGTAATAA54192.0272267134536177No Hit
AGAAGGGTTAGTTATAGGGGAGGATGTGGTATAGGTGTTTATGGGATTTT51851.9396882283183259No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG48991.8326967464863027No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTTTAGTAGTAGAATTATTTTGGT47811.7885534078283347No Hit
TGGTTTTTATTTTTGTGTTTTTTGGGGATGGTTTTTAAGGATAGTATGTT46661.7455323574413324No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATC43571.6299366655319085No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTCGGAGGAGAGG35781.3385158111712574No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGTGTTTATTTTTTTT34891.3052212591326207No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGT34571.2932501842423245No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATCGTTTTATTAGGATAGTT34141.2771640523584888No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGGGTTCGTTGGGGTTT33561.2554664791198267No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTATGTTGGAGT33501.2532219025778961No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGCGTGAAGGTT31551.180273164965153No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTATGGGATAGGGAGTAT30631.145856324655551No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTTGATTTTTTTGATT30481.1402448833007246No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTAGTTTTATTTTTAAATT30251.1316406732233242No Hit
AATTTGTTGTGGGTGATTATGGTGTATGGAGTTAGTGTGGATTTGTGTGG29481.1028352742685485No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTACGTATTAGTAGAGGTTTTTTG29421.100590697726618No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAAGGTATTGTGG27771.0388648428235276No Hit
TTTGGAGAGGAGGTTTTTAGATTTTATTTTGGTGGTATAGTGTTTTTTTT26781.0018293298816734No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTACGATAAGGTATGTTA25680.9606787599462797No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGTGTTGAT25360.9487076850559835No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGTGTGAAGAAGTGG25050.9371107062560089No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTT24210.905686634668981No Hit
TTTTTAGGTGAGATTAGGGATTTGGGTGTGGGGGGCGGTTAAAGGTATTT23410.8757589474432402No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTTGGAAGTATGGTATTT22130.8278746478820549No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGTGTTATTTTGTGATGGGTTAG20770.7769975795982956No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTAATTAATTGAGAAGTTGTT19940.7459476041015896No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGGGTTTGTTGGGGTTT19600.7332283370306497No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTAATTAATTGAGAAGTTGT19140.7160199168758488No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGCGTGAAGAAGTGG15790.5906977266180591No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTCGGGGCGTAGGCGGCGTTGAT15450.5779784595471192No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTACGTTGGAGT14260.5334610247988298No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATACGTTGTTGGTTGTTTTGA13780.5155044124633854No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGTGGGGGGTAT12720.4758502268892788No Hit
AGGTATTAGGGAGTTGTATGGGGTTGGTTTTGATTTTGTAGTTTAGACCA12340.4616345754570519No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATTGTTTTATTAGGATAGTT12120.4534044614699732No Hit
GTTGGGGATTGAGGTTTTTAGAGTGGAGGAGTTAGTTGTGGGTATTAGTG12110.45303036537965147No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTCGATTTTTTTGATT11960.447418924024825No Hit
TGGGTTGTGATTAGATGGTAGAAAGGGGTTTTAGTTAGGTTGATAATGGT11640.43544784913452866No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTAATTAATTGAGAAGTTGTTT11040.4130020837152231No Hit
TTTTTTTTGGGTTTTTAGGGTAGAGTTCGGGTTGGGGAGGTTAGTGTTTC11030.4126279876249013No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGCGTAGGCGGTGTTGAT10920.408512930631362No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAC10550.3946713752894569No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGTGGGGGGTAT10000.37409609032176006No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTG9940.3718515137798295No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTT9670.36175091934114195No Hit
GGGGATATTTAGGTTTGTTTTTTTTTGGGTTTGGTGTTTTTGGAGTTTTG9600.35913224670888966No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTTATTTTATGATGAGTTAG9570.3580099584379244No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTATGGGATAGGGAGTATT9320.34865755617988037No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGTGTGAAGGTT8630.3228449259476789No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGTTT8630.3228449259476789No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTTGGTGATTGTTTCGGAGGAGAGG8550.31985215722510485No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTTATTTTTTTTAGGTTTT8530.3191039650444613No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTTGTGATGGGTTAG8470.31685938850253076No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTTTT8150.30488831361223445No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAT8070.30189554488966036No Hit
GGGTTTAGGTTGTTGAGTTTTGGTGGGAAGGGTATTTTTTTTCGTTTATC7390.2764570107477807No Hit
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGTGGGTA7380.27608291465745893No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTATTTTTTTTAGGTTTTA7310.2734642420252066No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGTGTTATTTTGTGGTGGGTTAG7210.26972328112198896No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTAGTTTTATTTTTAAATTT6790.2540112453284751No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTTATTTTACGATGAGTTAG6670.24952209224461397No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTAATTAATTGAGAAGTTG6620.24765161179300513No Hit
GGGGATATTTAGGTTGTAAGATAGAGTGGGGTTTTAGACCAAGTCTCTGC5870.21959440501887317No Hit
GATAGGTTTGGAGATAGTTTTGTTTTTGTGGTTTTTTAGGGTTTGGTAGT5720.21398296366404676No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGCGGGGGGTAT5710.213608867573725No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGCGGGGGGTAT5500.20575284967696805No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGCGTTATTTTGTGATGGGTTAG5190.19415587087699346No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGCGGTGTTGAT4930.1844293725286277No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTCGCGATGGGTTAG4740.17732154681251427No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTTGGAGGAGAGG4600.1720842015480096No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATATGTTGTTGGTTGTTTTGA4530.1694655289157573No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTT4440.16609866410286148No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATT4350.16273179928996562No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTAATTAATTGAGAAGTTGTTTT4100.1533793970319216No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGTGTTATTTTGTGATGGGTTAG4080.1526312048512781No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAAGGTATTGCGG4060.1518830126706346No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTATTTTTTTTAGGTTTTAT3820.14290470650291232No Hit
GAAGTTTTGGGTGAAGTTAATTTTTATTTATTATTTTTGTTTTTTTTAAT3690.13804145732872947No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3500.130933631612616No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTTTT3490.13055953552229424No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTGTT3410.12756676679972018No Hit
TGGTTTTTATTTTTGTGTTTTTTGGGGATGGTTTTTAGGGATAGTATGTT2910.10886196228363217No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTTAGTTTTATTTTTAAAT2800.10474690529009281No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGCGTTGAT2760.10325052092880578No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTTTATTTTTTTTAGGTTT2750.10287642483848403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)