FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267311
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT81593.0522500009352402No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC75102.809461638316418No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT74902.8019797165099827No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA63002.3568053690270885No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG62762.3478270628593663No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC61442.2984463789368936No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA54432.03620501962134No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA53482.000665891040773No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC51581.9295876338796383No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT51361.9213575198925594No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA51261.9176165589893421No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT50451.8873147756732793No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA49911.8671135867959043No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC46661.7455323574413324No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC46631.744410069170367No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG46511.739920916086506No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA43531.6284402811706216No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA43451.6254475124480476No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA42581.5929011525900543No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT41331.5461391412998344No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC39681.4844132863967439No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA39491.4773054606806304No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC30511.14136717157169No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC30391.1368780184878289No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC29031.0860009502040695No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC28961.083382277571817No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT28951.0830081814814954No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA27921.044476284178354No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC27801.039987131094493No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA27721.0369943623719189No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA27351.0231528070300138No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA26921.007066675146178No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA26170.9790094683720459No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT24670.9228950548237821No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC24600.9202763821915297No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC23720.8873559262432149No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA22010.8233854947981938No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT20710.7747530030563651No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA17940.6711283860372375No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC17940.6711283860372375No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT16250.60790614677286No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA15840.592568207069668No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA14080.5267272951730381No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC13410.5016628571214803No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC13380.500540568850515No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT13220.4945550314053668No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGATC12830.4799652838828181No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA11140.4167430446184407No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC10710.400656912734605No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT9430.35277261317341974No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA9300.34790936399923683No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG8930.3340678086573317No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC8810.3295786555734706No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC8700.32546359857993123No Hit
AACCACTCACCTACTTAATCTCCCAATTCCAATATATCCAAAACTTACCC7760.2902985660896858No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT7630.2854353169155029No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT7130.2667305123994149No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC6890.2577522062316927No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAAATCCTATAACTCTAA6210.232313672089813No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC5870.21959440501887317No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC5760.21547934802533378No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATACCGCC5560.20799742621889858No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCGAAAAAAAAAACGACACA5390.20163779268342866No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA5360.20051550441246338No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT5300.19827092787053283No Hit
TACCACCTAAATCTCCAACTTCTCCAAAAAAACCAACTATCCTAATAAAA5260.19677454350924575No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA5010.18742214125120177No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC4950.18517756470927121No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA4920.18405527643830596No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC4630.1732064898189749No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCGCC4510.16871733673511377No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4230.1582426462061045No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC3960.148142051767417No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAACCCCGCC3810.14253061041259057No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCCAAAAATTTCTAAAAT3790.14178241823194707No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCA3590.13430049642551184No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA3590.13430049642551184No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAGCCCCCACCACCCGCAA3570.13355230424486833No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA3230.1208330371739285No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAATACCTAAAATAAA2780.10399871310944929No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATCCACACCAACATA2690.10063184829655344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)