FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262042
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTTTGGAGATAGTTTTGTTTAAAGATATTTAGTATTTTAGTGGTA71582.731623174910892No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG68122.5995832729104498No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAACGGTTT62972.403049892765282No Hit
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAGTATTGAGGTTAGGTTTG62252.3755733813663458No Hit
GGTTGTAGGAGGTTTTTAGTAAGGGAGGGGTTGTAGGAGTGTTTTTGGGG59902.2858931011059296No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA59322.263759244701231No Hit
GGTGGGGAATAAGGTTTTGTATTTATTGAGTTTTTGTTATAGGTTTGGGT59062.253837171140504No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATAGATGAGGTTGGGATGTAAGGAGA57452.192396638706772No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTTTAGTAGTAGAATTATTTTGGT56882.1706444005159478No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGTGTTGAT56342.150037016966746No Hit
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGCGGGTA55722.126376687706551No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATC55402.1141649048625792No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGCGGGGTAATAA53892.0565405545675883No Hit
AGAAGGGTTAGTTATAGGGGAGGATGTGGTATAGGTGTTTATGGGATTTT53362.036314789232261No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGTGGGGGGTAT52902.0187603513940515No Hit
TGGTTTTTATTTTTGTGTTTTTTGGGGATGGTTTTTAAGGATAGTATGTT51321.9584646736019415No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTT49931.90541974187344No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGGTTTTTTGT48771.861152029064043No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTCGGAGGAGAGG46261.765365857381641No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATCGTTTTATTAGGATAGTT44781.7088863617282724No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGTGTGAAGAAGTGG42381.6172979903984857No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGCGTGAAGGTT42311.614626662901367No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGTGTTATTTTGTGATGGGTTAG40221.5348684562016777No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTTGATTTTTTTGATT35741.3639034963860754No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTATGGGATAGGGAGTAT35631.3597056960334604No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGGGTTCGTTGGGGTTT34491.3162012196518116No Hit
GGGGTGTATTTATTAGTTTTAGGGATGTATTTTTAGGTTTCGGGGGTTTA31331.1956098640675923No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTAGTTTTATTTTTAAATT30461.1624090794605446No Hit
TTTTTAGGTGAGATTAGGGATTTGGGCGTGGGGGGCGGTTAAAGGTATTT28701.0952442738187007No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTTGGAAGTATGGTATTT27181.0372383053098357No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTACGTTGGAGT25400.9693102632402439No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGTGTTTATTTTTTTT25220.9624411353905099No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTTATTTTGCGATGAGTTAG24880.9494661161187902No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAAGGTATTGTGG24230.9246609322169728No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTATGTATTAGTAGAGGTTTTTTG22910.8742873279855901No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTATGTTGGAGT22250.8491005258698987No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTAATTAATTGAGAAGTTGT20840.795292357713649No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTAATTAATTGAGAAGTTGTT20070.7659077552453424No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGT19070.7277459338579312No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTGTGTAGAGGGTTTGTTGGGGTTT18690.7132444417307149No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTT15440.5892185222216285No Hit
TAGGTTGTTTTGGTTTAGAGAGGGCGGGAGTTTTTGTAGGATAATATAGT13880.5296860808572672No Hit
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGTGGGTA13280.5067889880248204No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGCGTTTATTTTTTTT13260.5060257515970722No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAT13230.5048808969554499No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGCGGGGGGTAT12890.49190587768373006No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTCCGAGCCTGGCTCCTTCCCACC12820.48923455018661133No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTACGATAAGGTATGTTA11840.45183596522694836No Hit
TTTGGAGAGGAGGTTTTTAGATTTTATTTTGGTGGTATAGTGTTTTTTTT11830.4514543470130743No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTCGAATTTTTTTTTTTTGT11490.4384793277413544No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTAATTAATTGAGAAGTTGTTT11450.436952854885858No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATATGTTGTTGGTTGTTTTGA10940.4174903259782783No Hit
TTTTTTTTGGGTTTTTAGGGTAGAGTTCGGGTTGGGGAGGTTAGTGTTTC10470.3995542699261951No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTATGATAAGGTATGTTA10380.39611970600132806No Hit
TTTGGAGAGGAGGTTTTTAGATTTTATTTTGGCGGTATAGTGTTTTTTTT10320.3938299967180834No Hit
TGGGTTGTGATTAGATGGTAGAAAGGGGTTTTAGTTAGGTTGATAATGGT10300.3930667602903351No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGTTT9100.3472725746254417No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTTTT9010.34383801070057474No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTATGGGATAGGGAGTATT9000.3434563924867006No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTCGAATTTTTTTTTTTGTT8830.3369688828508407No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTTGTTTGAGGTTTTTTGT8440.32208577250975035No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTACGTATTAGTAGAGGTTTTTTG8260.3152166446600163No Hit
GGGGATATTTAGGTTTGTTTTTTTTTGGGTTTGGTGTTTTTGGAGTTTTG8010.30567618931316354No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTTATTTTTTTTAGGTTTT7560.2885033696888285No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTAATTAATTGAGAAGTTG7250.27667320505873105No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTT7000.2671327497118783No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTCAAGGTATTGCGG6910.2636981857870112No Hit
TGGGATATTTTTGATTTATATTTGGGTTTAGGGAGTCAAGGTATTGCGGG6830.2606452400760183No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATACGTTGTTGGTTGTTTTGA6480.24728860259042446No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTATTTTTTTTAGGTTTTA6430.2453805115210539No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTG6240.23812976545744577No Hit
GGGGATATTTAGGTTTGTTTTTTTTTAGGTTGTAAGATAGAGTGGGGTTT6140.23431358331870464No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT5650.21561429083887315No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTTGTGATGGGTTAG4860.18546645194281833No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTAATTAATTGAGAAGTTGTTTT4720.18012379694858077No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTAGTTTTATTTTTAAATTT4460.17020172338785386No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAC4340.16562230482136453No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTTTT4040.15417375840514116No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTCGTTTATCG3800.14501492127216248No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTATTTTTTTTAGGTTTTAT3710.14158035734729546No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTCGAATTTTTTTTTTTTTG3630.13852741163630256No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTCGAATTTTTTTTTTGTTT3450.13165828378656858No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTTATGGGATAGGGAGTA3400.129750192717198No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGCTTGGAGTGGGGGCGTCGGGTA3170.12097297379809342No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTATTTTTTTTAGGTTTTATAA2770.10570824524312897No Hit
GAAGTTTTGGGTGAAGTTAATTTTTATTTATTATTTTTGTTTTTTTTAAT2750.10494500881538073No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTTTATTTTTTTTAGGTTT2700.10303691774601019No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTGTT2630.10036559024889138No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)