FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262042
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT74182.830843910518161No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT69262.6430877492920986No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA65342.493493409453446No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG62642.390456491707436No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACTAAAAAATAAACCCCTA62292.3770998542218424No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC61322.3400828874760533No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT54722.0882148663191398No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA54372.0748582288335458No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA53912.0573037909953364No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC53652.04738171743461No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA52822.0157074056830586No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT51781.9760191114401509No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC51381.9607543828851863No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA49531.8901550133184757No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC49511.8893917768907273No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC47731.8214637348211355No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA42561.6241671182482198No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT41801.5951641339937872No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA41751.5932560429244167No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC40421.54250082047916No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA39851.5207485822883355No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA37951.4482411216522542No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC34621.321162256432175No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA30331.157448042680181No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC30261.1547767151830624No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA30221.1532502423275657No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT29071.1093641477320428No Hit
CAAAACAAACAACACTAAAACCAAACTAACAAAAAACAAATCCCGCATCG28801.099060455957442No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA28731.096389128460323No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA27291.041436105662451No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA26521.0120515031941444No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC26020.9929705925004387No Hit
AACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTAATTTATAC25970.9910625014310683No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA25850.9864830828645789No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC25070.9567168621823983No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC24500.9349646239915738No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT24480.9342013875638256No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC23160.8838277833324428No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA22820.8708527640607231No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA21920.8365071248120531No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT19930.7605651002511048No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT19920.7601834820372306No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA16190.6178398882621869No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC15190.5796780668747759No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC14970.5712824661695454No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA14030.5354103540653788No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC13910.5308309354988895No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC12680.4838918951923737No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC11870.45298081986857064No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT10500.40069912456781737No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTACCCAAATAAATAACT8370.31941444501263155No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT7340.280107768983598No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA7230.2759099686309828No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA7010.26751436792575234No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC6770.2583555307927737No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC6450.2461437479488021No Hit
AACCACTCACCTACTTAATCTCCCAATTCCAATATATCCAAAACTTACCC6280.2396562383129422No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT6240.23812976545744577No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT5900.22515474618572595No Hit
AAAACCCCACTCTATCTTACAACCTAAAAAAAAACAAACCTAAATATCCC5770.22019370940536248No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA5650.21561429083887315No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT5630.21485105441112493No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC5030.19195396157867822No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5010.19119072515093No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAAACAACA4950.18890101586768535No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCATCCACTCT3910.1492127216247777No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC3770.14387006663054014No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA3730.1423435937750437No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC3640.1389090298501767No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA3000.11448546416223354No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG2650.10112882667663962No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)