FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272547
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT91303.3498809379666628No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC80722.961691011091665No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT76972.8241000634752904No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA66622.444349048054097No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA64502.36656429900164No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA61242.246951901873805No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA55472.0352452971414103No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA55382.0319431143986177No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA55072.020568929395664No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC53021.9453525446987123No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC51811.900956532267829No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG51661.895452894363174No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC49591.8195026912789354No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA48511.7798764983654194No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC48091.7644663122323858No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA47311.7358473951281796No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC45601.673105923015113No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA43431.5934866279944375No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC40151.4731404124793155No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG40141.4727735032856717No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT39401.4456222229560407No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC38071.3968233002014332No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT36791.349858923415044No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC36771.3491251050277566No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC36761.348758195834113No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA35501.3025276374350112No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA34941.281980722590966No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT34241.2562970790359094No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA33891.2434552572583812No Hit
TACCACCTAAATCTCCAACTTCTCCAAAAAAACCAACTATCCTAATAAAA33261.2203399780588302No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA32511.1928217885355554No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC27581.0119355560692285No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA27190.9976260975171255No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC27040.9921224596124705No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC26570.9748777275112184No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA25210.9249780771756798No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC25070.9198413484646685No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT24160.886452611843095No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAGAAAAAAAACAACAT22400.8218765937618099No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC19900.7301492953508937No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC19600.7191420195415836No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA17460.6406234521018392No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA17400.6384219969399774No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT15400.5650401582112443No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC15090.5536659732082907No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA14480.5312845123960271No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC11910.43698884962960516No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT11890.43625503124231785No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA10060.36911064880552713No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACACTA9310.34159245928225224No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA8740.3206786352445633No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC8380.3074699042733914No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC6410.23518879312558935No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA6000.2201455161861991No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5740.21060587715146378No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA4910.18015241407903956No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA4500.1651091371396493No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACGACACA4470.16400840955871832No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT4130.15153349697483368No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC3770.13832476600366175No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACGACACAA3490.12805130858163913No Hit
AAACCTATCAAAACCTCCCCACAAATCTCTATCTTCCACCCTAATGTAGA3370.12364839825791515No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG3370.12364839825791515No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACTAAACGCT3180.11667712357868551No Hit
CCCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA3130.11484257761046719No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA2990.10970584889945588No Hit
CACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA2820.10346839260751356No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC2790.10236766502658257No Hit
CTACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC2790.10236766502658257No Hit
CAACTACCAAACCCTAAAAAACCACTCTACACTAACCGAAACCCAAAAAA2740.10053311905836426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)