FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249288
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT70992.847710278874234No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC62602.511151760213087No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT59192.374362183498604No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA58012.3270273739610414No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG56822.27929142196977No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA53922.1629601103944034No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT51552.0678893488655694No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC51162.0522447931709507No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA50922.042617374281955No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC44321.7778633548345686No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT44251.7750553576586119No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC44021.765829081223324No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC42661.711273707519014No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA42201.6928211546484389No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA41871.6795834536760694No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT40221.613394948814223No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC39851.598552678027021No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA38231.5335676005262988No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC37161.4906453579795258No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG36701.4721928051089503No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA36171.4509322550624177No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA35461.4224511408491383No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC34881.399184878534065No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC33791.3554603510798755No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT33391.339414652931549No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA30431.2206764866339335No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC26901.0790732004749526No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA26721.071852636308206No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA26651.0690446391322486No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT25831.0361509579281794No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA25751.0329418182985142No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA25731.0321395333910979No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA23110.9270402105195598No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC22630.907785372741568No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT21340.8560379962132152No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC21080.845608292416803No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA19010.7625718044992138No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC17510.7024004364429897No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC16190.6494496325535124No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA14710.5900805494047046No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT14550.583662270145374No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA14280.5728314238952537No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA13720.5503674464875967No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT13650.5475594493116396No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA12990.521084047366901No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT12790.5130611982927377No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC12320.49420750296845417No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC11580.46452296139405025No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA11190.448878405699432No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC10080.4043515933378261No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA9430.3782773338467957No Hit
ACTCCCACACACACTAACACAATAAAACGACACTACGAACATCAATACAC9430.3782773338467957No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT8660.3473893649112673No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC7900.31690253842944704No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA7850.3148968261609063No Hit
CAACTACCAAACCCTAAAAAACCACTCTACACTAACCGAAACCCAAAAAA7620.3056705497256186No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT7390.29644427329033085No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT7090.28440999967908603No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACACTA7060.28320657231796154No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC7010.2812008600494208No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA6360.2551266005583903No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA6160.24710375148422709No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCCATCCTATAA5920.2374763325952312No Hit
CCACACTCCTAAAAACCTAACTTCCACGCACAAATTTCCCACACTACACC5270.21140207310420076No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA5200.20859407592824364No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA5170.20739064856711917No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4900.19655980231699882No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA4830.19375180514104168No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC4450.17850839190013157No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC4350.17449696736304998No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT4240.1700844003722602No Hit
AAACTACAAAATCAAAACCAACCCTACCCTATCTAACCTCAATTTCCCCA3920.15724784185359905No Hit
CCATAAAAACAAAAACCTAAAAAAAACTGAAACACCTATTAACCTATCCC3800.15243413240910111No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCAACCCCTAA3540.14200442861268894No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC3440.13799300407560733No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG3430.13759186162189918No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC3380.13558614935335836No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCGCACTCCCTTCTAACACC3380.13558614935335836No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCGAAACCCAAACGCCTCCAT3270.1311735823625686No Hit
TACCACCTAAATCTCCAACTTCTCCAAAAAAACCAACTATCCTAATAAAA3210.12876672764031963No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA3180.12756330027919516No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACC3070.12315073328840538No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTCACCAATCCACTC3020.12114502101986457No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC2930.11753473893649112No Hit
AAAAAAAATCCCAAAACAAACATACAAAATCAAAAAAATACCTAAAATAA2900.11633131157536665No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC2860.114726741760534No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCACCAAACCCCCACAAAAC2860.114726741760534No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT2750.11031417476974423No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA2720.10911074740861974No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC2720.10911074740861974No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAAATAACCAAATAAATAACT2590.10389589551041366No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)