FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245447
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT69482.830753686131833No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC64372.6225621009831044No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG61552.5076696802160954No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA58302.3752582023817768No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT56472.3007003548627605No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA54062.202512151299466No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT53962.198437951981487No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA53572.182548574641369No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC50302.049322256943454No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA48111.9600972918797133No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC47211.923429498017902No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC47021.9156885193137418No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC46611.8989843021100277No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC46221.8830949247699098No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC45221.8423529315901193No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG44421.809759337046287No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT41131.6757181794847766No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA38491.5681593174901303No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA37591.5314915236283189No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA37171.514379886492807No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC37151.5135650466292112No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC36101.4707859537904313No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT35251.4361552595876095No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA32331.3171886395026218No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA31991.303336361821493No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT30931.2601498490509153No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA28971.1802955424185262No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA26871.0947373567409664No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA25801.0511434240385908No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA25571.041772765607239No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA24721.0071420714044172No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC24641.003882711950034No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC24420.9949194734504803No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT23170.9439919819757422No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA21340.869434134456726No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT20280.8262476216861482No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCGCAAAAC18740.7635049521892711No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA16500.6722428874665406No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC15430.628648954764165No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA15110.6156115169466321No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT14170.5773140433576291No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC12930.5267939718146891No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT12910.5259791319510934No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC12740.519052993110529No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA10930.44530998545510847No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA10770.43879126654634193No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT10330.4208647895472342No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT9870.40212347268453064No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC9340.3805302162992418No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA8480.3454921021646221No Hit
AAAAAAAATCCCAAAACAAACATACAAAATCAAAAAAATACCTAAAATAA8450.3442698423692284No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT8120.33082498461989757No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC8040.32756562516551435No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA7800.31778754680236465No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATCCCACACCAACAT7620.3104539880300024No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA7240.2949720306216821No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA7220.2941571907580863No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA6860.2794900732133618No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA6090.2481187384649232No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC5470.22285870269345315No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC5100.20778416521693072No Hit
AACCACTCACCTACTTAATCTCCCAATTCCAATATATCCAAAACTTACCC5100.20778416521693072No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC5020.2045248057625475No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA4840.19719124699018523No Hit
CATAATCACCCACAACAAATTTCCCGCCGCCAAAATAAACCACCCCCTAA4800.19556156726299365No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC4760.193931887535802No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA4310.17559799060489636No Hit
AAAAAAATCCTTCCAACCAATAAAACTAACAATACCTACCCAACATGTAG3960.16133829299196975No Hit
CCATAAAAACAAAAACCTAAAAAAAAACTAAAACACCTATTAACCTATCC3910.15930119333298023No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACACCAAAAA3880.15807893353758654No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA3560.14504149572005362No Hit
ACCCCCAAAAAAACCTACTCATATAACCAAACACCACCTTTTCCCCAATA3060.12467049913015844No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG2980.12141113967577523No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC2930.1193740400167857No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC2880.1173369403577962No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAAACAACAC2640.1075588619946465No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC2550.10389208260846536No Hit
CCAACTAAAACTCAACATAATATCAAACAGAACAAAACGCACACTTCAAT2460.10022530322228425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)