FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239435
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT89363.732119364336876No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT78223.2668573934470735No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC64712.702612400025059No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA64612.6984359011840375No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG64142.678806356631236No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT60332.519681750788314No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT60162.5125817027585775No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA59042.465804915739136No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA57252.39104558648485No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC53242.2235679829598847No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA52492.192244241652223No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA50682.1166496126297325No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT50632.1145613632092215No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA49412.0636080773487584No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA48582.0289431369682793No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACTAAAAAATAAACCCCTA47962.0030488441539456No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT46021.9220247666381272No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA45971.9199365172176166No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA45741.9103305698832669No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC38541.6096226533297138No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC38021.5879048593564014No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA35921.5001983836949486No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA35401.4784805897216364No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA33801.4116566082652913No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT32911.3744857685801992No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA31791.3277089815607577No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCGATCCACTC27841.1627372773404054No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC27461.1468665817445236No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC26941.1251487877712114No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA26331.0996721448409799No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC25841.079207300519974No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA23510.9818948775241716No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC23340.9747948294944347No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC22230.9284356923590954No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA18880.7885229811848727No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC18380.7676404869797648No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC18080.7551109904567002No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC16390.6845281600434355No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCGAAACCCAAACGCCTCCAT14550.6076805813686387No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTCACCAATCCACTC13080.5462860484056216No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC12280.512874057677449No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC11700.48865036439952386No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAGAAC8190.3420552550796667No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTGATAAAA7690.3211727608745588No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6560.2739783239710151No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC6370.2660429761730741No Hit
AAAAATCCTAAAAACCCCAAAACACAAATTCCAAAATCTACCTAATGTAG5920.24724873138847703No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA5400.22553093741516486No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT5210.21759558961722386No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACC4560.19044834715058367No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA4170.17416000167059953No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAGCATCAATACAC3990.1666423037567607No Hit
CCCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA3860.16121285526343265No Hit
AAAACAAAAAAAACTCCTAATCCCTCCTACGAATTTCTATATTCAAATAT3600.15035395827677658No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT3420.14283626036293776No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCACCAAAAAAACCC3400.14200096059473344No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA3390.14158331071063127No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAACAATACCC3300.13782446175371188No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT3280.13698916198550756No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC3250.13573621233320107No Hit
AATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3130.1307244137239752No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC3060.1278008645352601No Hit
CAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT2990.12487731534654499No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCGAACGCT2780.11610666778039969No Hit
CCCAAAAATTAATAAATAAACCACTCACCTACTTAATCTCCCTGTAGAAC2630.10984191951886733No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAATACCTAAAATAAA2440.10190657172092635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)