FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275018
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT84403.0688900362885336No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC72772.646008624890007No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT65942.3976612439913025No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT64592.3485735479132277No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC63232.299122239271611No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG60072.184220669192562No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA56522.0551382091354022No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA55072.0024143874219145No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA53501.9453272149459306No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC52581.9118748591001316No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT51421.8696958017293412No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA51321.8660596760939285No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC49831.8118814041262754No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA49791.8104269538721103No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC49361.7947916136398345No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT48001.7453403049982184No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA46501.6907984204670239No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA43471.5806238137140116No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC41701.5162643899672021No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG41351.5035379502432566No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA37011.3457300976663347No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT36871.3406395217767564No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC35051.2744620352122407No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC33701.2253743391341658No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC33091.2031939727581467No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC32681.1882858576529536No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA31801.1562879520613196No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT31701.1526518264259067No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC30311.1021096800936665No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA30211.0984735544582536No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA27631.0046615130645995No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT27360.9948439738489844No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACAAACATCAATACAC26440.9613916180031853No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC25910.9421201521354967No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC25850.9399384767542488No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA23770.8643070635376594No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA22750.8272185820564472No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA20720.7534052316575642No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT20100.730861252718004No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA19680.7155895250492695No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA17170.6243227716004043No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC16790.6105054941858351No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA15770.573417012704623No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC15510.5639630860525493No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC14920.5425099448036128No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA14570.5297835050796675No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA13010.47305994516722544No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC12680.4610607305703627No Hit
TACCACCTAAATCTCCAACTTCTCCAAAAAAACCAACTATCCTAATAAAA12010.4366986888130958No Hit
CCTAAAAAAACAACACTCCAAACACGTATACCCGAACCCTAAATCCCGCC11850.43088088779643513No Hit
AATACCTAAAAACCCAAAACCCTTCTAAATAAAAAACCCTATAAAACTTC11210.4076096837297922No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA10900.3963376942600121No Hit
AAACTACAAAATCAAAACCAACCCGAAACCGCGCCAAACCCCCACAAAAC10880.3956104691329295No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA10740.39051989324335135No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCACCAAACCCCCACAAAAC10110.3676123017402497No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA8960.32579685693300076No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT8420.3061617785017708No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA7320.2661643965122283No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC7160.26034659549556755No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCGAAAAAAAAAACAACACA6720.24434764269975054No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCGATTCACCCA6670.24252957988204407No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT6340.23053036528518134No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA5960.2167130878706121No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC5610.20398664814666678No Hit
TAAAACTAAACCCCACATACCTTCCTAAACCCCTCCCAACCTCCCACCAC5570.20253219789250157No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGTACACTTCAAT5390.19598717174875827No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT5330.1938054963675105No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACAACCTTAAAAAACAATACC5290.1923510461133453No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4960.18035183151648254No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA4290.15598978975921576No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG4180.15199005156026152No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC4040.14689947567068337No Hit
AAAACAAAAAAAACTCCTAATCCCTCCTACAAATTTCTATATTCAAATAT3920.14253612490818784No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA3910.14217251234464653No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCGAAAAAAAAACAACACAA3820.13889999927277485No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT3670.13344581081965542No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA3450.12544633442174694No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA3390.12326465904049916No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA3350.12181020878633399No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA3190.11599240776967326No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA3080.111992669570719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)