FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238876
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT76973.222173847519215No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT70862.9663926053684757No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC61332.567440847971332No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG59052.471993837807063No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT57322.399571325708736No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA57192.3941291716204223No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA55882.3392890034997236No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA54922.299100788693716No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT54672.288635107754651No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA53062.2212361225070745No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA52062.1793733987508164No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC51942.1743498719000653No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA51332.1488136104087476No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC49922.089787169912423No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG49482.0713675714596693No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC48302.0219695574272842No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA48182.016946030576533No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC47922.006061722399906No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT47481.9876421239471525No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA45491.9043353036721982No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT42061.7607461611882318No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA39801.6661364054990875No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCAATCCACTC39641.659438369698086No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT37191.5568746964952527No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA36431.5250590264404962No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA35021.4660325859441719No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA30331.2696964115273195No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA26941.1277817779936032No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA26871.1248513873306654No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC26851.12401413285554No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC26331.1022455165022857No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC25901.0842445452870946No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA24371.020194577940019No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC23740.9938210619735762No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA21460.8983740518093069No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC18000.7535290276126526No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC17780.7443192283862757No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC16320.6831996517021383No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC14590.6107771396038112No Hit
AAAACAAAAAAAACTCCTAATCCCTAAACAATCTGTAGAACCATGTCGTC14260.5969624407642459No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC13920.5827291146871181No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA13800.5777055878363669No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAG11810.4943987675614126No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA11250.47095564225790787No Hit
CTCATTCAAAAAACCCAAAAAACCGAACCCAACTCATTCCACACCAACAT11110.4650948609320317No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC7040.29471357524405967No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC6380.2670841775649291No Hit
AATACCTTAACTCCCTAAAACCCAAAAATAACTAAAATAACTAAAATAAC5570.23317537132235971No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAACCATGTCGTCAG4920.20596460088079172No Hit
AATACCTAAAAACCCAAAACCCTTCTAAATAAAAAACCCTATAAAACTTC4790.20052244679247808No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA4240.17749794872653596No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3800.15907835027378223No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC3720.15572933237328151No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA3550.1486126693347176No Hit
CCTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC2930.12265778060583735No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT2870.12014601718046183No Hit
CAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT2560.1071685728160217No Hit
AATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC2480.10381955491552103No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)