FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264222
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC62412.362028899940202No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT61932.3438623581685096No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG54312.0554685075428996No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT54072.0463852366570534No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA54032.044871358176079No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT53832.0373019657712077No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA53522.0255694075436566No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA50781.9218687315969147No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT50491.910893112609851No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA49351.8677475759020825No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC47981.8158972379287115No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT47151.784484259448494No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA46981.7780502759043533No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA44781.694786959450765No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC42331.6020619024910872No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC42021.5903293442635362No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC40431.5301526746448062No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC38981.4752745797094866No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC38171.4446185404697565No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA37451.4173687278122187No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA37241.4094208657871032No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA35341.3375116379408225No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC35001.3246436708525406No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA33281.2595468961706444No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA32921.2459219898418754No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA31131.178175927818274No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC31031.174391231615838No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT30281.1460060100975693No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC30191.1425997835153772No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA29631.1214054847817365No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC28131.0646350417451993No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT27461.0392775771888791No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC26641.0082430683289052No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC26250.9934827531394056No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA25350.959420487317483No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA23960.906813210103625No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA23190.8776710493448692No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG21740.8227929544095496No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT21420.8106819265617549No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT21340.8076541695998063No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCACAAAAC19690.7452066822596151No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA18580.7031965544125773No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC17750.6717835759323599No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC15400.582843215175118No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC15200.5752738227702462No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT13990.5294789987207726No Hit
CCAACATATACAAACACAAAATACTCAACGACTACCTACACAAACAAACC13250.5014722468227475No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC12400.469302329102043No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA12240.4632468151781456No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT12030.45529895315303043No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCGTTCCACACCAACAT11390.4310768974574411No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC11170.4227505658120823No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT10800.40874718986306974No Hit
CAAAACAAACAACACTAAAACCAAACTAACAAAAAACAAATCCCGCATCG10790.40836872024282606No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT10710.40534096328087743No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA10280.3890667696104034No Hit
CAAAACAAACAACACTAAAACCAAACTAACAAAAAACAAATCCCGCATCA10200.38603901264845475No Hit
CATAATCACCCACAACAAATTTCCCGCCACCAAAATAAACCACCCCCTAA9820.37165716707919855No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA9740.36862941011724987No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC9460.35803226075042954No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC9220.34894898986458356No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA8590.3251054037892378No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCGCC8000.30277569619486644No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC6840.2588732202466108No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC6490.24562678353808542No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT5570.21080757847567574No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC5430.20550900379226558No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCGAAAAAAAAAACAACACA5170.19566879366593243No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4850.1835577658181378No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT4660.1763668430335097No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC4460.16879745062863802No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA4380.16576969366668937No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCCAACCGAAACCCAAAAAA4340.16425581518571505No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA4300.16274193670474071No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACACTACCTT4290.16236346708449712No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA3790.14343998607231798No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA3720.1407906987306129No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCACTCTAACCACCCTCG3400.12867967088281823No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA3380.12792273164233106No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT3060.1158117037945364No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC3020.11429782531356207No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCGAAAAAAAAACAACACAA2970.11240547721234416No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACC2780.10521455442771609No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCGATCCACTC2730.10332220632649816No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC2690.10180832784552384No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA2680.10142985822528025No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT2660.10067291898479308No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)