FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256917
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT75702.9464768777465093No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC61032.3754753480696102No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT60922.3711938096739416No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG55882.175021504999669No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA55522.1610091975229353No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA55412.156727659127267No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA53752.0921153524289946No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT50771.9761245849826987No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT50431.9628907390324504No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC50191.9535492007146278No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA48321.8807630479882609No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC47131.834444587162391No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA45811.7830661264143672No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC45671.7776168957289709No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA45151.7573768960403555No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC44671.7386938194047106No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC43951.7106692044512433No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC41641.6207568981422016No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA40851.590007667846036No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT39591.540964591677468No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA36571.4234169011782016No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT36281.412129209044166No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC32891.2801799803049234No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA32711.2731738265665564No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT32291.2568261345103673No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG31661.2323045964260833No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA30961.205058442999101No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA30641.1926030585753375No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA30171.1743092127029353No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC29021.129547674930036No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT28231.09879844463387No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC28061.0921815216587458No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA27111.0552045991506986No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT25220.9816399848978464No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC24980.972298446580024No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC24370.9485553700222251No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC23440.912356909040663No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA18410.7165738351296332No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC17880.6959446046777753No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC17840.6943876816248049No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA17620.6858246048334676No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA17580.6842676817804972No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA17540.6827107587275268No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC17310.6737584511729469No Hit
TACCACCTAAATCTCCAACTTCTCCAAAAAAACCAACTATCCTAATAAAA16060.6251046057676214No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC13920.5418092224337043No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATGCAC13650.531299991826154No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA13630.5305215302996688No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA13050.5079461460315977No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA12470.4853707617635267No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC11950.4651307620749114No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC10780.41959076277552676No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA9240.35964922523616577No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA8980.34952922539185804No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT8950.34836153310213025No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC8760.34096614860052077No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA8250.32111537967514797No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG7810.3039892260924734No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT6700.26078461137254444No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA6470.25183230381796456No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT6210.2417123039736569No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC5000.19461538162130182No Hit
AATACCTTAACTCCCTAAAACCCAAAATAACTAAATTAACCAAAAATAAC4760.18527384330347935No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC4640.18060307414456808No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4400.1712615358267456No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACACTA4360.1697046127737752No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA4140.1611415359824379No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA4120.16036307445595271No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC4060.15802768987649707No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG3480.13545230560842608No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT3350.13039230568627222No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA3220.12533230576411836No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATCCCACACCAACAT3220.12533230576411836No Hit
CATAATCACCCACAACAAATTTCCCACTACCAAAATAAACCACCCCCTAA3180.12377538271114796No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA2990.1163799982095385No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA2930.11404461363008288No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA2890.11248769057711244No Hit
CCTAAAAAAACAACACTCCAAACACTCCAAACCCCACTCTATCTTACAAC2750.107038459891716No Hit
CCATAAAAACAAAAACCTAAAAAAAAACTAAAACACCTATTAACCTATCC2570.10003230615334914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)