FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261255
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT71492.7364069587184936No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC69272.651432508468737No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA59092.2617748942603972No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA58872.2533540027942047No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG58052.2219670436929437No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA56942.1794798185680655No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC53162.03479359246713No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA49571.8973799544506325No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT48851.8698206732885494No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT47891.8330749650724387No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT46561.7821668484813689No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA46301.7722148858395053No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA46281.77144935025167No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA45811.7534592639375322No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC44761.7132686455761614No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC44671.7098237354309007No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG43841.6780540085357218No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC43691.6723124916269545No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA43231.6547051731067348No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC42871.6409255325256933No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC41581.5915484871102943No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT39061.495091003043004No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC38881.4882011827524833No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA37031.4173891408776866No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT35381.354232454881246No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT34171.3079175518171902No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA32421.2409331878815717No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC32371.2390193489119825No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA32111.2290673862701191No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC28581.0939503550171288No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA27331.0461043807774013No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC25690.9833304625748789No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC24030.9197910087845209No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT22830.8738588735143824No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC21010.8041951350213393No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT20550.7865878165011195No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA19880.7609423743086257No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA19590.7498421082850089No Hit
TACCACCTAAATCTCCAACTTCTCCAAAAAAACCAACTATCCTAATAAAA17020.6514707852481292No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC16770.6419015904001837No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA16520.6323323955522382No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT16480.630801324376567No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT16100.6162561482076898No Hit
AACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTAATTTATAC15170.5806587433733326No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC15050.5760655298463188No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCAGCAA14970.5730033874949761No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCACAAAAC14820.5672618705862089No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC13980.5351093758971119No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA13360.5113777726742071No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC13360.5113777726742071No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTCAAAACCCCCACCACCCGCAA13110.5018085778262616No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT11210.42908269698187596No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC10370.3969302022927791No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA10290.39386805994143653No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC9710.371667527894203No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA9000.34449101452603775No Hit
ACTCCCACACACACTAACACAATAAAACAACACTACGAACATCAATACAC8820.33760119423551704No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA8570.3280319993875715No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT7460.28554477426269353No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA7400.28324816749918663No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA7380.282482631911351No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC7260.2778894183843372No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA7230.2767411150025837No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATCAATTTATAC7180.27482727603299456No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA6420.24573692369524025No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCGATCCACTC6190.2369332644351304No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5420.2074601443034583No Hit
TAAAACTAAACCCCACATACCTTCCAAACCTATACCAAAAATTACCCCAA4730.18104916652312875No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA4590.1756904174082793No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG4250.1626763124150734No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC4100.1569347955063061No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT3740.1431551549252646No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC3700.14162408374959332No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAATACCTAAAATAAA3490.1335859600773191No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACAAAAAAAATAATCACA3080.11789248052668849No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA2960.11329926699967464No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA2850.10908882126657864No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA2800.10717498229698952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)