FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268861
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC67142.4972011559876663No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT65422.4332275785629007No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA58572.1784490870747337No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT57642.1438587225369243No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA57612.142742904326027No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA55782.0746779934613055No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA55632.06909890240682No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA54212.0162835070910248No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA54062.010704416036539No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC51851.9285058078337878No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC51791.9262741714119938No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC50751.887592473434228No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC50441.876062351921625No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG50331.871971018481669No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA47391.762620833813755No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA46181.7176161659742395No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC43671.6242593756625172No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC43261.6090098601135903No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC36951.374316096421571No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT36001.3389818530764968No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA35871.3341466408292761No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA35851.3334027620220112No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT35331.3140619130331286No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT34921.2988123974842019No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC34721.2913736094115547No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA34181.271288881615407No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT33701.253435790241054No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC31341.165658090983817No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT30141.1210253625479336No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA29951.113958513878919No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA28931.076020694708418No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG27271.0142787537054463No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC26390.9815480861857986No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA25870.9622072371969159No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA25240.9387750547680772No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA22220.8264493548711045No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT20230.7524334135482648No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC20210.751689534741No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA19690.7323486857521173No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC18660.6940389271779842No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCGCC18030.6706067447491455No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC17960.6680031689237189No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT17790.6616801990619688No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA17280.6427112894767184No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT16600.6174194100297179No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA16180.6017979550771588No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA15360.5712989239793053No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC14930.5553055296231137No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA14910.5545616508158491No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT14480.5385682564596577No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC14160.5266661955434221No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC13580.5050937101327452No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT13100.4872406187583919No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA13000.48352122472206827No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCGCACCAACAT12130.4511624966060529No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA12050.4481869813769941No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT11950.4444675873406705No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC10790.40132261651931667No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA9780.3637567367524483No Hit
ACCCCCAAAAAAACCTACTCATAGGCGCTTAGAGCCCCCACCACCCGCAA9480.35259855464347745No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCA8740.32507503877468286No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC8480.31540461428024147No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC8240.3064780685930648No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6590.24510806699372537No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT6280.2335779454811222No Hit
AACCACTCACCTACTTAATCTCCCAATTCCAATATATCCAAAACTTACCC5510.20493861140143046No Hit
TAAAACTAAACCCCACATACCTTCCAAATCATAAAACACAACCAATTCCT5330.198243702136048No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC5220.19415236869609204No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC4880.18150642897259178No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT4370.16253751938734143No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA3790.14096503397666452No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG3690.13724563994034092No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA3500.13017879127132606No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA3330.12385582140957595No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA3250.12088030618051707No Hit
CCTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC3220.11976448796961998No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA3170.11790479095145819No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTCATT3120.11604509393329637No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT3070.1141853969151346No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAGACCCCTA3000.11158182108970806No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAATACCTAAAATAAA2860.10637466943885503No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA2690.10005169957710491No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)