FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000888120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238609
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAGTATTGAGGTTAGGTTTG58622.4567388489118183No Hit
AGAAGGGTTAGTTATAGGGGAGGATGTGGTATAGGTGTTTATGGGATTTT57942.428240342987901No Hit
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGCGGGTA54692.29203424849859No Hit
GGTGGGGAATAAGGTTTTGTATTTATTGAGTTTTTGTTATAGGTTTGGGT51622.16337187616561No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAACGGTTT51382.1533135799571683No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTT48862.0477014697685334No Hit
TAGGTTGTTTTGGTTTAGAGAGGGTGGGAGTTTTTGTAGGATAATATAGT48852.047282374093182No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTTTAGTAGTAGAATTATTTTGGT47982.0108210503375816No Hit
GGTTGTAGGAGGTTTTTAGTAAGGGAGGGGTTGTAGGAGTGTTTTTGGGG47631.9961527017002711No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATC47301.982322544413664No Hit
TGGTTTTTATTTTTGTGTTTTTTGGGGATGGTTTTTAAGGATAGTATGTT47171.9768743006340919No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG46201.9362220201249742No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGCGGGGTAATAA45331.8997606963693743No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTCGGAGGAGAGG43941.8415063974954842No Hit
GATAGGTTTGGAGATAGTTTTGTTTAAAGATATTTAGTATTTTAGTGGTA42621.7861857683490565No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATAGATGAGGTTGGGATGTAAGGAGA42361.7752892807899114No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA39251.6449505257555248No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGCGTGAAGGTT38801.6260912203646969No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTTATTTTACGATGAGTTAG36441.5271846409816898No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTATGGGATAGGGAGTAT34331.4387554534824756No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAAGGTATTGTGG32931.3800820589332339No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTATGTTGGAGT32221.3503262659832613No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATCGTTTTATTAGGATAGTT31381.3151222292537164No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGGGTTCGTTGGGGTTT29671.2434568687685712No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGTGTTTATTTTTTTT27531.153770394243302No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTTGATTTTTTTGATT25691.0766567899785842No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTACGATAAGGTATGTTA25641.0745613116018256No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGGTTTTTTGT25551.07078945052366No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTAGTTTTATTTTTAAATT25301.0603120586398669No Hit
GGGGTGTATTTATTAGTTTTAGGGATGTATTTTTAGGTTTCGGGGGTTTA24781.038519083521577No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGTGTTGAT24651.0330708397420048No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTTGTTTGAGGTTTTTTGT24551.0288798829884875No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGTGGGGGGTAT23680.9924185592328872No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGT23550.9869703154533149No Hit
TTTGGAGAGGAGGTTTTTAGATTTTATTTTGGTGGTATAGTGTTTTTTTT22760.9538617571005283No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGTGTTATTTTGTGATGGGTTAG22190.92997330360548No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGTGTGAAGAAGTGG21920.9186577203709835No Hit
TTTTTAGGTGAGATTAGGGATTTGGGCGTGGGGGGCGGTTAAAGGTATTT21620.9060848501104317No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTAATTAATTGAGAAGTTGT19570.8201702366633279No Hit
AATTTGTTGTGGGTGATTATGGTGTATGGAGTTAGTGTGGATTTGTGTGG18470.774069712374638No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTT18190.7623350334647897No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTTGGAAGTATGGTATTT16620.6965370124345687No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTAATTAATTGAGAAGTTGTT15950.668457602186003No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGCGTTGAT15220.6378636178853271No Hit
TTGTGTGATGGGGATAGTTTATTTTAGAGAGTAAGTTGGTAAAGATTTAT14600.61187968601352No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGCGTGAAGAAGTGG14450.6055932508832441No Hit
GGGGATATTTAGGTTTGTTTTTTTTTGGGTTTGGTGTTTTTGGAGTTTTG13730.5754183622579199No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATACGTTGTTGGTTGTTTTGA13320.5582354395684991No Hit
TGGGTTGTGATTAGATGGTAGAAAGGGGTTTTAGTTAGGTTGATAATGGT11920.49956204501925744No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGCGTAGGTGGTGTTGAT11800.4945328969150367No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTGTGTAGAGGGTTCGTTGGGGTTT11210.46980625206928495No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATTGTTTTATTAGGATAGTT10270.43041125858622264No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGTTT9080.38053887321936725No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTGTGTAGAGGGTTTGTTGGGGTTT8990.37676701214120173No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT8870.371737864036981No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTTGGGTA8430.35329765432150506No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAT8360.350363984594043No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGCGGGGGGTAT8040.33695292298278773No Hit
TTTTTTTTGGGTTTTTAGGGTAGAGTTCGGGTTGGGGAGGTTAGTGTTTC8020.3361147316320843No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTAATTAATTGAGAAGTTGTTT7970.33401925325532567No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTAATTAATTGAGAAGTTG7550.3164172348905531No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTTGATTTTTTTGGTT7500.3143217565137945No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGCGTTTATTTTTTTT7480.3134835651630911No Hit
TAGGTTGTTTTGGTTTAGAGAGGGCGGGAGTTTTTGTAGGATAATATAGT7450.3122262781370359No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTCGT7130.29881521652578064No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTACGTATTAGTAGAGGTTTTTTG7020.2942051640969117No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTTATTTTTTTTAGGTTTT7010.29378606842155996No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAAGGTATTGCGG6870.2879187289666358No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTATGGGATAGGGAGTATT6780.28414686788847027No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTT6660.27911771978424954No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTG6590.2761840500567875No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGCGTTATTTTGTGATGGGTTAG6570.27534585870608397No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGTGTTATTTTGTGATGGGTTAG6170.258582031692015No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTTTT6150.2577438403413115No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGCGGGGTAACAA6140.2573247446659598No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTCGTTTATCG5990.2510383095356839No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTATTTTTTTTAGGTTTTA5930.24852373548357354No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATAGACGAGGTTGGGATGTAAGGAGA5820.24391368305470454No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTCGATTTTTTTGATT5160.21625336848149065No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTCGTGGCGGGGGGTAT5000.20954783767586305No Hit
GGGTTTAGGTTGTTGAGTTTTGGTGGGAAGGGTATTTTTTTTCGTTTATC5000.20954783767586305No Hit
TTTTTAGGTGAGATTAGGGATTTGGGTGTGGGGGGCGGTTAAAGGTATTT4800.2011659241688285No Hit
AGGTATTAGGGAGTTGTATGGGGTTGGTTTTGATTTTGTAGTTTAGACCA4660.19529858471390432No Hit
GATAGGTTTGGAGATAGTTTTGTTTTTGTGGTTTTTTAGGGTTTGGTAGT4630.19404129768784917No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTATGTATTAGTAGAGGTTTTTTG4490.18817395823292501No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATATGTTGTTGGTTGTTTTGA4400.18440209715475947No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGTGGGGGGTAT4320.18104933175194565No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTCGTT4230.17727747067378014No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGCGGTGTTGAT3790.1588372609583042No Hit
AATTTGTTGTGGGTGATTATGGCGTATGGAGTTAGTGTGGATTTGTGTGG3390.14207343394423513No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTATTTTTTTTAGGTTTTAT3280.13746338151536613No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTAGTTTTATTTTTAAATTT3220.13494880746325577No Hit
TTTGGAGAGGAGGTTTTTAGATTTTATTTTGGCGGTATAGCGTTTTTTTT3210.13452971178790407No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTT3090.12950056368368335No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTGTT3090.12950056368368335No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTTATGGGATAGGGAGTA3020.12656689395622128No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGACGTTGAGTGTGAAGAAGTGG2980.12489051125481436No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTAATTAATTGAGAAGTTGTTTT2970.12447141557946265No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTTTATTTTTTTTAGGTTT2970.12447141557946265No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTTTT2970.12447141557946265No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGACGTTGAGCGTGAAGAAGTGG2880.12069955450129709No Hit
GGGGATATTTAGGTTTGTTTTTTTTGGGTTTGGTGTTTTTGGAGTTTTGT2820.11818498044918674No Hit
GAGTATGTGGTTTTTTTATGTTGGGTATATTGTTATTTATGTTTACGTTT2540.1064503015393384No Hit
GGTGGGGAATAAGGTTTTGTATTTATTGAGTTTTTGTTATAGGCTTGGGT2540.1064503015393384No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGTGTCATTTCGCGATGGGTCAG2430.10184024911046943No Hit
TTTTTTTTGGGTTTTTAGGGTAGAGTTCGGGTTGGGGAGGTTAGTGTTTT2430.10184024911046943No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTACGTTGGAGT2390.10016386640906251No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)