FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238609
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT68192.85781341022342No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT59452.491523789966011No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA55082.308378979837307No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC54772.2953870139014034No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC51882.174268363724755No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA51382.1533135799571683No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA50252.1059557686424233No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC49832.088353750277651No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT48522.0334522168065745No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA44411.8612038942370155No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA44301.8565938418081462No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA41881.7551726883730288No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG41611.7438571051385319No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC40751.7078148770582835No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA40631.7027857289540629No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA40181.6839264235632354No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC36891.5460439463725173No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC36771.5410147982682967No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA36661.5364047458394277No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC36611.534309267462669No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT36051.5108399096429723No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC35681.4953333696549584No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT34741.4559383761718963No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA32321.3545172227367785No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC29891.2526769736263093No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT29841.2505814952495504No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT29751.246809634171385No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA29681.2438759644439228No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA28021.1743060823355365No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA27491.152094011541895No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC27101.1357492802031777No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC26561.1131181137341843No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA23850.9995431857138667No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA23730.994514037609646No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT23270.9752356365434666No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC22970.9626627662829148No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC20170.8453159771844314No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT17410.729645570787355No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA16740.7015661605387894No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA16650.6977942994606239No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC16340.6848023335247203No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT15660.656303827600803No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA15300.6412163832881408No Hit
CACCCAAAATACCTCAACTACCTTATTCTAATCTAACAAACGTTATCTCC14820.621099790871258No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC14270.5980495287269131No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC13600.5699701184783473No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA13200.5532062914642785No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT12360.5180022547347334No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT10490.43963136344396064No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAGCACC9720.40736099644187773No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA9690.4061037094158225No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT9670.40526551806511907No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC8990.37676701214120173No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA8930.37425243808909137No Hit
AAACCTATCAAAACCTCCCCACCAGAAACCTACCGCTCTAACCACCCTCG8840.37048057701092585No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG8160.34198207108700845No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC7940.33276196622927046No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA7920.331923774878567No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC7350.30803532138351863No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC6990.2929478770708565No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA5920.2481046398082218No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5890.24684735278216663No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA5820.24391368305470454No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA5080.2129006030786768No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC4980.20870964632515956No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC4940.20703326362375266No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCGATCCACTC4820.20200411551953196No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCCAATAAAA4710.19739406309066299No Hit
TACTATCCACACTCTCTTACCTCCCGACCCCCGCACTCCCTTCTAACACC4620.19362220201249744No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA4480.18775486255757326No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAAATCCTATAACTCTAA4120.17266741824491114No Hit
TACCACCTAAATCTCCAACTTCTCCAAAAAAACCAACTATCCTAATAAAA4060.17015284419280077No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3310.13872066854142132No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC3070.1286623723329799No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA3040.12740508530692474No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG2820.11818498044918674No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC2820.11818498044918674No Hit
AATACCTAAAAACCCAAAACCCTTCTAAATAAAAAACCCTATAAAACTTC2530.10603120586398669No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAAACAACAC2440.10225934478582115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)