FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249550
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTT72352.899218593468243No Hit
GGTGGGGAATAAGGTTTTGTATTTATTGAGTTTTTGTTATAGGTTTGGGT59802.3963133640553No Hit
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAGTATTGAGGTTAGGTTTG58972.363053496293328No Hit
GATAGGTTTGGAGATAGTTTTGTTTAAAGATATTTAGTATTTTAGTGGTA58802.356241234221599No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA57502.3041474654377883No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGCGGGGTAATAA57282.295331596874374No Hit
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGCGGGTA55802.236024844720497No Hit
TAGGTTGTTTTGGTTTAGAGAGGGCGGGAGTTTTTGTAGGATAATATAGT54992.2035664195551994No Hit
GGTTGTAGGAGGTTTTTAGTAAGGGAGGGGTTGTAGGAGTGTTTTTGGGG54872.198757763975155No Hit
AGAAGGGTTAGTTATAGGGGAGGATGTGGTATAGGTGTTTATGGGATTTT54652.189941895411741No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAACGGTTT54592.1875375676217192No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATC54382.179122420356642No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG51592.067321178120617No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATCGTTTTATTAGGATAGTT49761.9939891805249448No Hit
TGGTTTTTATTTTTGTGTTTTTTGGGGATGGTTTTTAAGGATAGTATGTT49531.9847725906631937No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGTGTTGAT41131.6481667000601083No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTATGGGATAGGGAGTAT40111.6072931276297335No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTTGATTTTTTTGATT39531.5840512923261871No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGTGTTATTTTGTGATGGGTTAG39341.5764375876577839No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAAGGTATTGTGG37841.516329392907233No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTATGTTGGAGT36411.459026247245041No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGCGTGAAGGTT35461.4209577239030255No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATAGATGAGGTTGGGATGTAAGGAGA34631.387697856141054No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTACGTATTAGTAGAGGTTTTTTG32451.3003406131035864No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGGTTTTTTGT31471.2610699258665599No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTAGTTTTATTTTTAAATT31331.2554598276898417No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTTGGTGATTGTTTCGGAGGAGAGG30941.2398316970546985No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTGTGTAGAGGGTTCGTTGGGGTTT29261.1725105189340814No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGT29091.1656982568623524No Hit
TTTTTAGGTGAGATTAGGGATTTGGGCGTGGGGGGCGGTTAAAGGTATTT28081.1252254057303146No Hit
GGGGTGTATTTATTAGTTTTAGGGATGTATTTTTAGGTTTCGGGGGTTTA26131.0470847525545983No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTTATTTTACGATGAGTTAG25391.0174313764776597No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTT24310.9741534762572631No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGTGTTTATTTTTTTT23760.9521138048487277No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGGGTTCGTTGGGGTTT22320.8944099378881987No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATACGTTGTTGGTTGTTTTGA21560.8639551192145862No Hit
TTTGGAGAGGAGGTTTTTAGATTTTATTTTGGTGGTATAGTGTTTTTTTT19240.7709877780004007No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTACGATAAGGTATGTTA18880.7565618112602684No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTTGTTTGAGGTTTTTTGT18160.727709877780004No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTAATTAATTGAGAAGTTGTT18070.7241033860949709No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGCGGGGGGTAT17880.7164896814265678No Hit
AATTTGTTGTGGGTGATTATGGTGTATGGAGTTAGTGTGGATTTGTGTGG17340.6948507313163694No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGTGTGAAGAAGTGG16690.668803846924464No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTAATTAATTGAGAAGTTGT15760.6315367661791224No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGCGTGAAGAAGTGG13520.541775195351633No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGTGGGGGGTAT12860.5153275896613905No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTCGTGGTGGGGGGTAT12590.5045081146062913No Hit
TGGGTTGTGATTAGATGGTAGAAAGGGGTTTTAGTTAGGTTGATAATGGT12190.4884792626728111No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTAATTAATTGAGAAGTTGTTT11500.4608294930875576No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTCGGAGGAGAGG11010.4411941494690443No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAC10530.4219595271488679No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGACGTTGAGTGTGAAGAAGTGG10340.4143458224804648No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTT9770.3915047084752554No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGCGGGGGGTAT9740.39030254458024444No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATGGATGAGGTTGGGATGTAAGGAGA9730.3899018232819075No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTG9440.3782809056301342No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTATGGGATAGGGAGTATT8480.3398116609897816No Hit
GGGGATATTTAGGTTTGTTTTTTTTTGGGTTTGGTGTTTTTGGAGTTTTG8360.33500300540973754No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTATTTTTTTTAGGTTTTA8300.33259867761971545No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTTATTTTTTTTAGGTTTT8260.33099579242636745No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGACGTTGAGCGTGAAGAAGTGG8190.3281907433380084No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTTTT8090.32418353035463837No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTATGATAAGGTATGTTA8020.32137848126627927No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTTGTGATGGGTTAG7960.31897415347625724No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTCGGGGCGTAGGCGGTGTTGAT7920.31737126828290924No Hit
TTTTTTTTGGGTTTTTAGGGTAGAGTTCGGGTTGGGGAGGTTAGTGTTTC7350.29453015427769985No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTAATTAATTGAGAAGTTG7310.29292726908435185No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAT7260.29092366259266683No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGTTT7240.29012221999599275No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTCTGGGGTAAGTGTTTATTTTTTTT7090.2841114005209377No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGCGTAGGTGGTGTTGAT6700.26848326988579446No Hit
AATTTGTTGTGGGTGATTATGGCGTATGGAGTTAGTGTGGATTTGTGTGG6010.240833500300541No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTCGGGGCGTAGGTGGCGTTGAT5450.2183931075936686No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTACGTTGGAGT5420.2171909436986576No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT5400.21638950110198354No Hit
GGGGTGTATTTATTAGTTTTAGGGATGTATTTTTAGGTTTTGGGGGTTTA4990.19995992787016628No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTATTTTTTTTAGGTTTTAT4900.19635343618513326No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTTTT4750.19034261671007816No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTAATTAATTGAGAAGTTGTTTT4490.17992386295331597No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGCGTTATTTCGTGATGGGTTAG4440.17792025646163093No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTT4080.16349428972149868No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGCGTAGGTGGTGTTTAT4060.1626928471248247No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTAGTTTTATTTTTAAATTT3900.1562813063514326No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTTTATTTTTTTTAGGTTT3670.14706471648968145No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTCGTTTATCG3460.13864956922460428No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTTATGGGATAGGGAGTA3180.1274293728711681No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTGGAGTGTTTGGAGTGTTGTTTTT2940.11781206171107994No Hit
TTTTTTTTGGGTTTTTAGGGTAGAGTTTGGGTTGGGGAGGTTAGTGTTTC2880.1154077339210579No Hit
GGGGATATTTAGGTTTGTTTTTTTTGGGTTTGGTGTTTTTGGAGTTTTGT2870.11500701262272091No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTTAGTTTTATTTTTAAAT2770.11099979963935082No Hit
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGCAAGGCGGGTA2660.10659186535764377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)