FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249550
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT74873.0002003606491687No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT65052.606692045682228No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA53722.1526748146663994No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA53622.1486676016830293No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT51192.0512923261871365No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG50732.0328591464636347No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC50192.0112201963534364No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG49651.9895812462432378No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC49531.9847725906631937No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA46521.8641554798637545No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA45371.818072530554999No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC44951.8012422360248446No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT44701.7912242035664196No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA43561.7455419755560007No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT43441.7407333199759567No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA43151.7291124023241837No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA38631.5479863754758565No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC37921.5195351632939291No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC37471.5015027048687637No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC37451.5007012622720897No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC35681.4297735924664396No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT35211.4109396914446004No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA34861.396914446002805No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA34281.3736726106992587No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA30721.2310158284912842No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC30551.2242035664195552No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT29901.19815668202765No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA27821.1148066519735524No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC27761.1124023241835304No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT26901.0779402925265478No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT26141.0474854738529353No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA25971.0406732117812063No Hit
ACTCCCACACACACTAACACAATAAAACAACACTACGAACATCAATACAC25451.0198357042676818No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA24330.9749549188539371No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA24070.9645361650971749No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC24050.9637347225005009No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC23430.9388900020036065No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC22790.913243838910038No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC21480.8607493488278902No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA19270.7721899418954117No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC16850.6752153876978562No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA16360.6555800440793428No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC13560.5433780805449809No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC12940.5185333600480866No Hit
CAACTACCAAACCCTAAAAAACCACTCTACACTAACCAAAACCCAAAAAA12590.5045081146062913No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC12130.486074934882789No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAACAA11420.45762372270086155No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC10660.42716890402724905No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC10470.41955519935884594No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA9590.38429172510518933No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC9490.38028451212181924No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT9160.36706070927669804No Hit
AAACTACAAAATCAAAACCAACCCGAAACCGCGCCAAACCCCCGCAAAAC8360.33500300540973754No Hit
TACTATCCACACTCTCTTACCTCCCGACCCCCACACTCCCTTCTAACACC8350.3346022841114005No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT8080.32378280905630136No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCGCC7770.31136044880785413No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATGCC7490.30014025245441794No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACC7250.2905229412943298No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA6680.26768182728912043No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA6480.2596674013223803No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCGATCCACTC6370.2552594670406732No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5680.22760969745541973No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA5400.21638950110198354No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA5230.20957723903025446No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA5200.20837507513524345No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA5170.2071729112402324No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA5120.20516930474854736No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCCAAAAT4970.19915848527349228No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC4730.18954117411340413No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA4720.1891404528150671No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC4540.181927469445001No Hit
TACCACCTAAATCTCCAACTTCTCCAAAAAAACCAACTATCCTAATAAAA3750.15027048687637748No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC3730.14946904427970345No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG3690.14786615908635542No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCACCAAAAAAACCC3680.14746543778801843No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT3650.14626327389300742No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACACTACCTT3180.1274293728711681No Hit
CCTAAAAAAACAACACTCCAAACACTCCAAACCCCACTCTATCTTACAAC3120.12502504508114606No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA3110.12462432378280905No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC3100.12422360248447205No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACACACACTTCAAT3050.12221999599278703No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC2910.11660989781606894No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT2800.11220196353436186No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC2780.11140052093768785No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCCACCGCTCTAACCACCCTCG2570.10298537367261071No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAACAAAAACACCTAACGCTAAA2550.1021839310759367No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)