FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254267
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT76503.0086483892915714No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT63282.4887224846322957No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC62602.461978943394149No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA55672.189430795187736No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA54762.1536416444131565No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA52922.081276768121699No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACTAAAAAATAAACCCCTA51732.034475570954941No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC49461.945199337704067No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC48651.9133430606409798No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA46061.8114816315133304No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT45751.7992897230077045No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT45201.7776589175944972No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG45071.7725461817695571No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC43161.6974282938800551No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA42971.68995583382822No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA41361.6266365670731946No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC38721.5228087010897995No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT38651.5200556894917547No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC36261.42606000778709No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT36041.417407685621807No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA35961.4142613866526133No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA35801.4079687887142256No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA33401.3135798196384116No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA32451.2762175193792353No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT32001.2585195876775201No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA31411.235315632779716No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA30221.188514435612958No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC29761.1704232165400936No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT29661.1664903428286015No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA29341.1539051469518262No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC28231.1102502487542623No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT27271.0724946611239368No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC26491.041818246174297No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC25120.9879378763268533No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC24200.9517554381811245No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA22450.8829301482300103No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC21240.835342376320954No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA19900.7826418685869578No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC19760.7771358453908687No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCGATCCACTC17820.700838095387919No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC16140.6347658170348492No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC15630.6147081611062387No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC15280.6009431031160158No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC14320.5631875154856902No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACACACACTTCAAT12930.5085205708959479No Hit
AAACTACAAAATCAAAACCAACCCGAAACCGCGCCAAACCCCCGCAAAAC12150.4778441559463084No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA12140.4774508685751592No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA11310.4448080167697735No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG10860.4271100850680584No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA10440.41059201547979096No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC9730.3826686121281959No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAACATCCCAAAAC9700.3814887500147483No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT9620.3783424510455545No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC9610.3779491636744053No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA9020.354745208776601No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA8910.3504190476939595No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT8330.3276083801673044No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTCAACTATATC7290.28670649356778505No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC7080.2784474587736513No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA6680.2627159639276823No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC6370.2505240554220563No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCGATCCACTC6120.2406918711433257No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC5780.22732010052425206No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCACTTACCAATCCACTC5520.2170946288743722No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA5210.20490272036874624No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC5180.20372285825529857No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4840.19035108763622494No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA4730.18602492655358344No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAAATCCTATAACTCTAA4020.15810152320198848No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT3690.14512303995406403No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA3650.14354989046946712No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA3510.13804386727337797No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC3460.13607743041763187No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT3420.13450428093303496No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG3340.13135798196384116No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC3220.12663853351005044No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCACCAAACCCCCACAAAAC3160.12427880928315511No Hit
TACCACCTAAATCTCCAACTTCTCCAAAAAAACCAACTATCCTAATAAAA3120.12270565979855821No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT2950.1160197744890214No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT2820.11090703866408146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)