FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280165
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT75102.680563239519569No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC64822.3136366069994465No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT62452.229043599307551No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT62072.215480163475095No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA61742.203701390252173No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA57832.0641407741866398No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA56902.030946049649314No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT56442.014527153641604No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC55471.979904699016651No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA54831.9570610176146201No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA53821.9210108329020397No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT52581.876751200185605No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG52131.8606892366998018No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA47371.6907893562721967No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA44441.586208127353524No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC43211.5423054271589955No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA41671.4873378187853588No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC40931.4609248121642604No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC39731.4180929095354522No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA37621.3427801474131316No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC37201.3277889814930488No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC35911.28174468616708No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACAAACATCAATACAC33961.212142844395267No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT31361.1193403886995164No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA31181.1129156033051952No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA30591.091856584512698No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA30161.0765084860707084No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA29831.0647297128477862No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC28611.0211839451751648No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC28091.0026234540360146No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG25490.9098209983402639No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT24300.8673460282333625No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC21230.7577677440079953No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC20960.7481305659165135No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA20220.7217175592954153No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC19750.7049417307657987No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACGCCAAACCCCCGCAAAAC19680.7024432031124516No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA19190.6849535095390217No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA18920.6753163314475399No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT18860.6731747363160994No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC18590.6635375582246177No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT18470.6592543679617369No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC16290.5814430781860689No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA16030.5721628326164938No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT15210.5428943658201417No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC15150.5407527706887013No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCGCACCAACAT14570.5200506844181108No Hit
TACCACCTAAATCTCCAACTTCTCCAAAAAAACCAACTATCCTAATAAAA14490.5171952242428569No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA12760.45544589795299195No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA11830.42225117341566576No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC11510.41082933271465033No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT11310.4036906822765156No Hit
AATAAAAAACTCCCTCTTCCAAAAAATCATTTAAAATTCAAAACCTTATT11210.4001213570574483No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA11000.39262577409740695No Hit
ACTCCCACACACACTAACACAATAAAACAACACTACGAACATCAATACAC10870.38798565131261936No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC10770.384416326093552No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC10520.3754930130458837No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCACTTACCAATCCACTC9560.3412274909428373No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC9400.33551657059232953No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC8760.31267288919029856No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA7610.2716256491710242No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA7540.2691271215176771No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA7240.2584191458604751No Hit
AAACTACAAAATCAAAACCAACCCGAAACCGCGCCAAACCCCCACAAAAC7140.25484982064140776No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC6990.24949583281280674No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT6840.2441418449842057No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT6630.23664626202416433No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT6230.222368961147895No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACACTA5080.18132172112862063No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC5010.1788231934752735No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT4940.17632466582192635No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4920.1756108007781129No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA4910.17525386825620617No Hit
ACTCCCTTTTCCCTTTAACTTTATTATACATCTCTCTAAAAATTTCTAAA4830.1723984080809523No Hit
AACCACTCACCTACTTAATCTCCCAATTCCAATATATCCAAAACTTACCC4710.16811521781807148No Hit
CCTAAAAAAACAACACTCCAAACACAACCCCACTCTATCTTACAACCTAA4680.1670444202523513No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAACACC4610.16454589259900415No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA4340.15490871450752236No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACGACACA4290.15312405189798867No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC4240.15133938928845503No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA4070.14527153641604054No Hit
CTCATTCAAAAAACCCAAAAAACCAAGCCCAACTCATTCCACACCAACAT4040.14420073885032034No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG4010.14312994128460016No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA3950.14098834615315975No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT3650.13028037049595775No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA3510.12528331518926347No Hit
AAACTACAAAATCAAAACCAACCCCTTACAAAATAAATCCTTACTATATA3180.11350454196634126No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACGACACAA3080.10993521674727393No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC2980.1063658915282066No Hit
CATAATCACCCACAACAAATTTCCCACCGCCAAAATAAACCGCCCCCTAA2820.10065497117769885No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)