FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252006
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT74152.9423902605493524No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC53462.1213780624270853No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT52572.0860614429815163No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA50942.021380443322778No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT49031.9455885970968947No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA49001.9443981492504148No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA48971.9432077014039348No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC47361.8793203336428497No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC47271.8757489901034103No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA46331.8384482909137085No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG45971.8241629167559503No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT44381.7610691808925185No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC43441.7237684817028167No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC43251.716228978675111No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT41951.6646429053276508No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA41521.6475798195281066No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA40481.6063109608501385No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC39111.5519471758608923No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC38021.5086942374387913No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG34731.3781417902748347No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA34321.3618723363729435No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA32111.2741760116822616No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC31921.2666365086545557No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC31651.2559224780362372No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT31311.2424307357761324No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC31181.2372721284413863No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT30841.2237803861812815No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA30071.193225558121632No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA28611.1352904295929462No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA27101.0753712213201272No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA24120.957120068569796No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA23170.9194225534312674No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA23040.9142639460965215No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC20170.8003777687832829No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT19390.769426124774807No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA19380.7690293088259803No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT17990.7138718919390807No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC16560.6571272112568748No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG16430.6519686039221289No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC16160.6412545733038102No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT15680.6222074077601326No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC14800.5872876042633906No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA13790.5472091934319024No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC12880.5110989420886805No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA12570.4987976476750554No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC12570.4987976476750554No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA12240.48570272136377707No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA11940.47379824289897865No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC11770.46705237176892617No Hit
TACCACCTAAATCTCCAACTTCTCCAAAAAAACCAACTATCCTAATAAAA10610.42102172170503877No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA8940.354753458250994No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC8720.3460235073768085No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA8540.33888082029792943No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA8440.33491266080966325No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA8240.32697634183313096No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA8230.32657952588430433No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA7750.3075323603406268No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC7360.29205653833638884No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT6680.26507305381617896No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC6530.2591208145837797No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAGCCCCCACCACCCGCAA6270.24880359991428774No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC6220.24681952017015468No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC6120.24285136068188853No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC6010.2384863852447958No Hit
CATAATCACCCACAACAAATTTCCCACCGCCAAAATAAACCACCCCCTAA5870.23293096196122318No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA5740.22777235462647713No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAACCACCAAAACCACAC5720.2269787227288239No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCGATTCACCCA5400.21428061236637222No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA5300.21031245287810607No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT5300.21031245287810607No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5230.2075347412363198No Hit
AAAAAAAATCCCAAAACAAACATACAAAATCAAAAAAATACCTAAAATAA5020.19920160631096084No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT4900.19443981492504148No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC4830.19166210328325517No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACGAACC4270.16944041014896472No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC4080.161900907121259No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC4010.1591231954794727No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT4010.1591231954794727No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA3960.15713911573533962No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA3940.1563454838376864No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA3770.14959961270763394No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC3640.14444100537288795No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCGATCCACTC3550.14086966183344843No Hit
CAACTACCAAACCCTAAAAAACCACTCTACACTAACCGAAACCCAAAAAA3550.14086966183344843No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG3490.13848876614048872No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC3400.1349174226010492No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCACTTACCAATCCACTC3400.1349174226010492No Hit
CATAATCACCCACAACAAATTTCCCGCCACCAAAATAAACCACCCCCTAA3330.13213971095926288No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCCTATT3250.12896518336864995No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAAATCCTATAACTCTAA3160.12539383982921043No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACGCAAACAAACC3080.1222193122385975No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAGACCCCCGCAAAAC3020.1198384165456378No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCACAAAAC2910.11547344110854503No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC2880.11428299326206519No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA2720.10793393808083934No Hit
AAAAATCCTAAAAACCCCAAAACACAAATTCCAAAATCTACCTAATGTAG2690.1067434902343595No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT2690.1067434902343595No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAACAACACAAA2670.10594985833670627No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAAACAACAC2630.1043625945413998No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCA2610.10356896264374657No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA2570.1019816988484401No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA2560.1015848828996135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)