FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246688
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCCCTTTTCCCTTTAACTTTATTATATACCTCTCTAAAAATTTCTAAA100624.078836424957841No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC88933.604958490076534No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC87593.550638863665845No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA85603.4699701647425085No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA85443.463484239200934No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC81093.2871481385393695No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA76363.095407964716565No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA74393.0155500064859253No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA65552.6572026203139187No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC64582.617881696718122No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC64442.612206511869244No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA62902.5497794785315864No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC62892.549374108185238No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC62702.541672071604618No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT59562.414385782851213No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG58682.3787131923725515No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT54132.1942696847840186No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT50712.0556330263328575No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA49171.9932059929952002No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA45871.8594337787002206No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT44471.8026819302114412No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC31481.2761058503048384No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC29891.211651965235439No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT27961.1334154883901932No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAGTCACCAAAACCGCAC24580.996400311324426No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC23680.9599169801530678No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT23260.942891425606434No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC22540.9137047606693476No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT18650.7560156959398107No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC18140.735341808276041No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT15280.6194058892203917No Hit
TACCACCTAAATCTCCAACTTCTCCAAAAAAACCAACTATCCTAATAAAA13590.5508983006875081No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC12450.5046860812037878No Hit
TAAACACCAAACCCAAAAAAATAAAAATTTAAATCACCCCACCAAAAAAA12420.5034699701647425No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA11910.4827960825009729No Hit
AACTTACACATCTCAAAAAAACCCAAAACTAAACCGAACTCAAAACTAAC11270.4568523803346738No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA10870.44063756648073676No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA10070.4082079387728628No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATACCTCTCTAAAAATTTCTAAA8750.3546990530548709No Hit
AAAAATCCTAAAAACCCCAAAACAAACAATACCTCTCCTACCCCCAAAAA7240.2934881307562589No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC6580.2667336878972629No Hit
CCTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA6480.2626799844337787No Hit
AAAAACCACAAAAACCTTCTCAATATTCGTAAAAAAACCCAATTCACCCA6250.25335646646776494No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG4070.16498573096380853No Hit
CTAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA4010.16255350888571798No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAAATCCTATAACTCTAA3630.14714943572447786No Hit
CACTCCATTATTCTTATCTTTAATCTCTTTTTATTTATAAAAATCCAATT3620.14674406537812945No Hit
AAAAAAATCCTTCCAACCAATAAAACTAACAATACCTACCCAACATGTAG3520.1426903619146452No Hit
CCTAAAAAAACAACACTCCAAACACCACTCTATCTTACAACCTAAATGTA3360.13620443637307045No Hit
CTACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC3150.12769165909975352No Hit
CAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG3050.12363795563626931No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAATACCTAAAATAAA3040.12323258528992087No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC2900.11755740044104294No Hit
CAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA2900.11755740044104294No Hit
CACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC2860.11593591905564925No Hit
ACTCCCACACACACTAACACAATAAAACAACTCTACGAACATCAATACAC2670.10823388247502919No Hit
AAAACAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACAA2580.10458554935789337No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)