FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000888142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239073
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAATATTGTTGTTTTTTAAGGTTTGCGTGTTATTTTGTGATGGGTTAG160966.73267161076324No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATCGTTTTATTAGGATAGTT131135.484935563614461No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTT123985.185863731998176No Hit
TAGGTTGTTTTGGTTTAGAGAGGGCGGGAGTTTTTGTAGGATAATATAGT119875.01394971410406No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGCGTAGGCGGCGTTGAT119334.991362470877096No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGCGTGAAGAAGTGG117674.921927612068281No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTACGTTGGAGT115764.842035696209944No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAACGGTTT104614.375650951801332No Hit
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGCGGGTA99994.182404537526195No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTCGGAGGAGAGG98264.110041702743514No Hit
TGGTTTTTATTTTTGTGTTTTTTGGGGATGGTTTTTAAGGATAGTATGTT98194.107113726769648No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAAGGTATTGTGG94783.964479468614189No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTTGTTTGAGGTTTTTTGT93983.9310168860557235No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTTGATTTTTTTGATT89363.737770471780586No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTAGTTTTATTTTTAAATT82013.4303329945246848No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTATGATAAGGTATGTTA45061.884779962605564No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTACGATAAGGTATGTTA31441.3150794945476905No Hit
GGGGATATTTAGGTTTGTTTTTTTTTGGGTTTGGTGTTTTTGGAGTTTTG28531.1933593504912725No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTCTCGGAAGTATGGTATTT27091.1331267018860347No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGTTT25211.054489632873641No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTTTT23290.9741794347333241No Hit
AGAAGGGTTAGTTATAGGGGAGGATACACTGACGACATGGTTCTACAAGA12160.5086312548886741No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTTTT11690.48897198763557576No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTAGTTTTATTTTTAAATTT10710.4479803240014556No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTGTT7930.33169784961078835No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTTAGTTTTATTTTTAAAT7580.3170579697414597No Hit
GGGGATATTTAGGTTTGTTTTTTTTGGGTTTGGTGTTTTTGGAGTTTTGT6160.2576618857001836No Hit
GATAGGTTTGGAGATAGTTTTGTTTTTGTGGTTTTTTAGGGTTTGGTAGT5430.22712727911558395No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGCGCAGGCGGCGTTGAT5210.21792506891200594No Hit
TTTAAGGTTTGTGTTGATGTTAATGTATAGGTTATGATAAGGTATGTTAG3980.16647634822836538No Hit
AGGTATTAGGGAGTTGTATGGGGTTGGTTTTGATTTTGTAGTTTAGACCA3130.1309223542599959No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTGTTTTTT3060.12799437828613017No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTCCGTTGATGAACGGTTT2860.11962873264651382No Hit
GGGGATATTTAGGTTTGTTTTTTTTTTGGGTTTGGTGTTTTTGGAGTTTT2830.11837388580057137No Hit
GGAAATATTGTTGTTTTTAAGGTTTGCGTGTTATTTTGTGATGGGTTAGA2650.11084480472491666No Hit
TTTAAGGTTTGTGTTGATGTTAATGTATAGGTTACGATAAGGTATGTTAG2490.10415228821322357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)