FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239073
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT138215.78107941925688No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT135075.649738782714904No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA117924.932384669117801No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC114184.775947095656975No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC110024.601941666352955No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA107364.490678579346057No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA97354.07197801508326No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT89733.753246916213876No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG89463.741953294600394No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA88393.6971970904284466No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC85443.5738038172441056No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC84153.51984540286858No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA81913.426150171704877No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA79773.336637763360982No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC78913.3006654871106313No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA78213.271385727371974No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC51402.149970929381402No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA24931.0427777289781783No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT24431.0218636148791373No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAACCATGTCGTCAG12960.5420938374471396No Hit
CCAACATATACAAACACAAAATACTCAACGACTACCTACGCAAACGAACC12500.522852852476022No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT11850.4956645041472688No Hit
ATACAAAACAAACCCCTATCTCCTCTGCCGCGACCTTAAAAAACAATACC11520.4818611888419018No Hit
CCAACATATACAAACACAAAATACTCAACGACTACCTAAGCAAACGAACC8910.37268951324490845No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA7360.30785575953788175No Hit
CAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT6610.2764845883893204No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG6570.27481145926139716No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA5220.2183433511939868No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAAATCCTATAACTCTAA5190.21708850434804433No Hit
CACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA4920.20579488273456226No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAGCCTTATT4530.18948187373731037No Hit
CTACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC4490.1878087446093871No Hit
CAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA4390.1836259217895789No Hit
CAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG3960.16563978366440377No Hit
TAATCTAAACCTCCTAACCTCAAACGCTCTATCTACTTCTACCTCCGCCT3890.16271180769053803No Hit
CACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA3740.15643757346082576No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA3520.1472353632572478No Hit
CAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA3330.13928799989961224No Hit
CCCCTAATAAAAACTTCCTTCCACTACCCACAAACATTAAAACCTTCATC3320.13886971761763145No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG3270.13677830620772735No Hit
CCATAAAAACAAAAACCTAAAAAAAAACTAAAACACCTATTAACCTATCC3140.13134063654197672No Hit
TAATCTAAACCTCCTAACCTCAAACTATCTATCTACTTCTACCTCCGCCT3130.1309223542599959No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA2980.12464812003028364No Hit
AAAACCCCACTCTATCTTACAACCTAAATACAAAACCCAAACACCTCCAT2970.1242298377483028No Hit
CATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC2860.11962873264651382No Hit
CATAACACAAACACAAAAAACCAAAAAAAAATCCTATAACTCTAAAAAAA2670.1116813692888783No Hit
CATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT2610.1091716755969934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)