FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280006
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT70022.5006607001278542No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT68192.435304957750905No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA67142.397805761305115No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC62772.2417376770497777No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC57962.0699556438076328No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT56472.016742498375035No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA55341.9763862202952793No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA54881.9599580008999806No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC54081.931387184560331No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA53721.9185303172074883No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT50511.8038899166446434No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC50501.8035327814403979No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG47041.6799640007714123No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT45941.6406791283043936No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA45821.6363935058534462No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA42321.5113961843674777No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC41391.4781826103726348No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC40101.4321121690249494No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG38011.3574709113376142No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT38001.3571137761333685No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC37061.32354306693428No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA35681.274258408748384No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA34831.243901916387506No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA34281.2242594801539968No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT33731.2046170439204873No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT33361.1914030413633994No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC32701.167832117883188No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA32651.16604644186196No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC32111.1467611408326965No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC28971.0346206866995709No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC28131.0046213295429385No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC26110.9324800182853225No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA25960.927122990221638No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA25750.91962315093248No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA23760.848553245287601No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA22390.7996257223059506No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC21820.77926901566395No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT21610.7717691763747919No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA20550.7339128447247558No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA19050.6803425640879124No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA18900.6749855360242281No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC17860.6378434747826832No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA17670.6310579059020164No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC17620.6292722298807882No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA16550.5910587630265066No Hit
CAACTACCAAACCCTAAAAAACCACTCTACACTAACCGAAACCCAAAAAA14260.5092748012542588No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC13790.49248944665471456No Hit
TACCACCTAAATCTCCAACTTCTCCAAAAAAACCAACTATCCTAATAAAA12150.4339192731584323No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC12000.428562245094748No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT11920.425705163460783No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC10830.38677742619801003No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC9770.348921094547974No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC9590.3424926608715528No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCGAAAAAAAAAACGACACA9570.3417783904630615No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA9230.3296357935187103No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC8880.31713606137011346No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC8780.3135647093276573No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA8460.30213638279179733No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCGAAAAAAAAACGACACAA7960.28427962257951617No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCACAAAAC7880.2814225409455512No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC7840.2799940001285687No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA7570.27035134961393686No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT7520.2685656735927087No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT7490.2674942679799719No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA7470.26677999757148063No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT7240.2585658878738313No Hit
CTCATTCAAAAAACCCAAAAAACCCAACTTTACTACTCCCTTTTCCCTTT7100.25356599501439253No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC5750.20535274244123342No Hit
CCTAAAAAAACAACACTCCAAACACACATACCCAAACCCTAAATCCCACC5660.20213852560302278No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTCAAAAAACAATACC4950.17678192610158353No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT4560.16285365313600422No Hit
AAAAATCCTAAAAACCCCAAAACCTACATCTAAAACCTTCTAACTACAAA4490.16035370670628488No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCACTCTAACCACCCTCG4370.15606808425533739No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA4330.1546395434383549No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA4300.153568137825618No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT4190.14963965057891618No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA4130.14749683935344243No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAGCACC4080.14571116333221432No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCGATCCACTC3900.13928272965579308No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA3890.13892559445154745No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC3730.13321143118361747No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC3660.13071148475389813No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT3600.12856867352842438No Hit
AACCACTCACCTACTTAATCTCCCAATTCCAATATATCCAAAACTTACCC3590.12821153832417875No Hit
ACTCCCACACACACTAACACAATAAAACAACACTACGAACATCAATACAC3580.12785440311993315No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCGATTCACCCA3550.12678299750719627No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA3520.1257115918944594No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC3200.11428326535859946No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT3180.11356899495010822No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAAATCCTATAACTCTAA3060.10928337249916074No Hit
ACTCCCACACACACTAACACAATAAACAACGCTACGAACATCAATACACT3000.107140561273687No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA2970.10606915566095013No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATGA2920.10428347963972201No Hit
TAATCTAAACCTCCTAACCTCAAACCATAAAAAAATCATCTACCCTCATG2840.101426398005757No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)