FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278841
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT86783.1121678662750454No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC65952.365147162719973No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA64522.313863456234915No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA62732.249669166299074No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC60902.184040367090923No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA58912.112673530793535No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT56772.0359272847249867No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG55581.9932506338737845No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA53881.932283989800639No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA50941.826847558285905No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA48631.7440046478100424No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC44481.5951743108079515No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG44091.5811878454029358No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA43261.5514217780025175No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC42851.536718057961347No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC40981.469654749480887No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA39681.4230331981308344No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT38981.3979292858654215No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA37551.3466455793803638No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA37501.344852442789977No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC37381.3405489149730492No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA37151.3323004866572707No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC37041.32835558615842No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA36761.3183140212522548No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC34241.2279399370967683No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT34091.2225605273256086No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA33571.2039119067855875No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT33321.1949462238336543No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA33101.187056422835953No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT33081.1863391681997986No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT31661.135414089032818No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT30941.1095929221312504No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC29721.0658403893258166No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT29361.0529298058750327No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC29271.0497021600123368No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC29201.0471917687857955No Hit
ACTCCCACACACACTAACACAATAAAACAACACTACGAACATCAATACAC28111.008101391115367No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTGTCCC27981.0034392359803614No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC24340.8728988922002144No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA22830.818746167170538No Hit
TAATCTAAACCTCCTAACCTCAAACGATTTATCTACTTCTACCTCCGCCT22540.8083459749462957No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC21460.7696142245939442No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA20770.7448689396466086No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC20030.7183305181088865No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC18220.6534189735368903No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC15550.5576654796102438No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC15110.5418858776148414No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC14010.5024368726263354No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA13470.48307099745015974No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA13410.48091923354169586No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT12620.452587675413587No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA12100.433939054873566No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT11870.42569062655778744No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT11570.4149318070154676No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACAAACATCAATACAC11030.3955659318392919No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC10210.3661584917569511No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA10060.36077908198579123No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA8060.2890536183703257No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC7660.2747085256472327No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAAACAACA7600.2725567617387687No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT6990.25068049533605175No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5940.21302462693793237No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC5860.21015560839331374No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC4740.1699893487686531No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACAAAAAAAATAATCACA4690.16819621217826647No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCCACCGCTCTAACCACCCTCG4090.14667857309362684No Hit
AAACTACAAAATCAAAACCAACCCCCTAATACCTTGTAGAACCATGTCGT4080.14631994577554952No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA4080.14631994577554952No Hit
CAACTACCAAACCCTAAAAAACCACAAACACTCAACACCAACCCATAAAA3960.14201641795862158No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTGTAACTCTAAA3880.13914739941400298No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT3530.12659544328129652No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA3480.12480230669090987No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA3260.11691250569320867No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA3200.11476074178474471No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC2900.10400192224242491No Hit
AAAAAAATCCTTCCAACCAATAAAACTAACAATACCTACCCAACATGTAG2800.10041564906165161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)